Disulfiram (DSF) was obtained from Sigma-Aldrich (PHR1690) and dissolved in 0.9% saline and 0.5% Tween 80. The DSF stock solutions were all sonicated for at least 30 minutes and briefly vortexed before each administration. DSF was administered intraperitoneally (50, 100, or 200 mg/kg). In Experiment 3, prazosin (α1-adrenergic receptor antagonist; 3 mg/kg) and propanolol (β-adrenergic receptor antagonist; 10 mg/kg) were obtained from Sigma-Aldrich (P7791; P0884) and dissolved in 0.9% saline solution. All solutions were vortexed before administration. All solutions were injected in a volume of 1mL/kg.
Phr1690
Manufactured by Merck Group
Sourced in United States
The PHR1690 is a precision laboratory device designed for accurate temperature measurement and control. It features a digital display, intuitive controls, and reliable performance to support various research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phr1690
1
Disulfiram Administration and Receptor Antagonists
2
Disulfiram Attenuates Lung Injury in a Mouse I/R Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All mice were randomly allocated to three groups: the sham group (n = 12), the I/R group (n = 12), and the DSF (disulfiram, a GSDMD inhibitor) + I/R group (n = 12). Mice in the DSF + I/R group were given intraperitoneal injections of disulfiram (50 mg/kg) (PHR1690, Sigma-Aldrich, St. Louis, USA) at 24 h and 4 h prior to the surgery. The LIRI model was established as previously described [36 (link)]. Mice were anesthetized with an intraperitoneal injection of pentobarbital (50 mg/kg). After orotracheal intubation, mice were ventilated using a rodent ventilator (TOPO, Kent Scientific, South Elgin, USA) throughout the procedure. Mice underwent a left thoracotomy, followed by the interruption of the left pulmonary hilum via a microvascular clamp. Subsequently, the clamp was removed following 60 min of left lung ischemia, initiating a 6-h period of lung reperfusion. At the end of the experiment, all mice were humanly euthanized via cervical dislocation. After that, bronchoalveolar lavage fluid (BALF) and lung tissues were collected for subsequent experiments.
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