Cell viability was measured by the CKK-8 assay (Sigma–Aldrich). Briefly, cells dispersed evenly in medium were seeded in a 96-well plate with a density of 1x104 cells/well. Next day, cells were treated with genistein and DSS for 4 days. After incubation, CKK-8 solution was added to each well, followed by a 2 h incubation. The optical density (OD) in 570 nm was measured by a BioTek multilabel counter.
Ckk 8 assay
Manufactured by Merck Group
The CKK-8 assay is a colorimetric method used to measure cell viability and cytotoxicity. It utilizes a tetrazolium salt that is reduced by metabolically active cells to produce a colored formazan product, which can be measured spectrophotometrically.
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Lab products found in correlation
3 protocols using ckk 8 assay
1
Cell Viability Assay with Genistein and DSS
2
Hydrogen Peroxide-Induced Oxidative Stress
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Intestinal porcine epithelial cells (IPEC-J2) were cultured in serial passage in uncoated plastic culture flasks (100 mm2) in DMEM-H containing 10% FBS, 5 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were treated with different dosage of H2O2 to induce oxidative stress. Cell viability was evaluated with the CKK-8 assay (Sigma–Aldrich) according to the manufacturer's instructions. Briefly, 8 × 103 cells were seeded in 96-well plates. The following day, cells were incubated with 50, 100, 200, 250, 300, 400, and 500 uM H2O2 for 4 hours and then assayed.
3
Cell Viability Assessment of Drug Combinations
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Cell viability after drugs treatment was tested by the CKK-8 assay
(Sigma–Aldrich). Briefly, cells were seeded in a 96-well plate
(1 × 104 cells/well). Next day, cells were incubated with
different concentrations of matrine (0, 10, and 100 µM) plus cisplatin (0.1,
1.0, and 5.0 mM), 5-fluorouracil (0.1, 1.0, and 10.0 mM), or paclitaxel (0.1,
1.0, and 5.0 mM) for 48 h. After treatment with drugs, cells were treated with
CKK-8 for 2 h incubation. Then, the optical density (OD) of each well was tested
using a microplate reader at 570 nm and the cell proliferation inhibitory ratio
was calculated basing on the formula:
(ODcontrol − ODtreatment/ODcontrol) × 100%.
(Sigma–Aldrich). Briefly, cells were seeded in a 96-well plate
(1 × 104 cells/well). Next day, cells were incubated with
different concentrations of matrine (0, 10, and 100 µM) plus cisplatin (0.1,
1.0, and 5.0 mM), 5-fluorouracil (0.1, 1.0, and 10.0 mM), or paclitaxel (0.1,
1.0, and 5.0 mM) for 48 h. After treatment with drugs, cells were treated with
CKK-8 for 2 h incubation. Then, the optical density (OD) of each well was tested
using a microplate reader at 570 nm and the cell proliferation inhibitory ratio
was calculated basing on the formula:
(ODcontrol − ODtreatment/ODcontrol) × 100%.
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