2 chloro l phenylalanine

Acetonitrile for UPLC grade was purchased from Thermo Fisher Scientific (81 Wyman Street, Waltham, MA, USA). Deionized water was produced using a Mill-Q ultrapure water system (Millipore, Burlington, MA, USA). Formic acid for UPLC grade was obtained from Tianjin Kermel Chemical Reagent Company (Tianjin, China). Diclofenac sodium (Sustained release tablets) for positive control group was purchased from Novartis (Beijing, China). Monosodium urate for GA model group was purchased from Sigma-Aldrich CN (Shanghai, China). 2-Chloro-l-phenylalanine for using as an internal standard (IS) was provided by Macklin Biochemical Company (Shanghai, China). P-b4 was extracted form Pulsatilla Chinensis by our research group (Jiangxi University of Traditional Chinese Medicine, Nanchang, China), the purity reached 98.50%.

Methanol and acetonitrile (analytical gradient grade) for lipid chromatography were purchased from Merck (Darmstadt, Germany). Deionized water was produced using a Mill-Q ultrapure water system (Millipore, USA). HPLC grade formic acid was obtained from Tianjin Kermel Chemical Reagent Company (Tianjin, China). 2-Chloro-L-Phenylalanine for use as an internal standard (IS) was provided by Shanghai Macklin Biochemical Company (Shanghai, China). Standard eicosapentaenoic acid, palmitoleic acid, glycocholic acid, gamma-Linolenic acid, arachidonic acid, thymidine, linoleic acid, L-Phenylalanine, L-Tryptophan, and taurocholic acid were purchased from Shanghai Yuanye Biotechnology Co., Ltd.