Spheroids were lysed in lysis buffer (0.5% TritonX-100, 150 mM NaCl, 20 mM Tris-HCl pH7.5). The lysates were sheared with a 21-gauge needle, incubated on ice for 30 minutes and clarified by centrifugation at 20,817 × g for 15 minutes at 4°C. The extracted proteins were separated by SDS-PAGE and transferred to immobilon transfer membrane (Millipore) for Western blotting analyses. The primary antibodies were anti-YAP1 pAb (#4912 Cell Signaling, 1:500), anti-Fibronectin pAb (F3648, Sigma Aldrich, 1:1000), anti-ARHGAP18 pAb (1:10000)17 (link),21 (link), anti-MYH9 pAb (#3403 Cell signaling, 1:1000), anti-Phospho Ser1943-MYH9 pAb (#5026 Cell Signaling, 1:1000), anti-MYH10 mAb (#8824 Cell Signaling, 1:1000), anti-Phospho-Ser19 MLC2 (#3675, Cell signaling, 1:100), and anti-GAPDH mAb (sc32233, Santa Cruz, 1:5000).
Anti myh9 pab
Manufactured by Cell Signaling Technology
Anti-MYH9 pAb is a polyclonal antibody that specifically detects the MYH9 protein. MYH9 is a non-muscle myosin heavy chain protein involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh mab, by Santa Cruz Biotechnology (2 mentions)
Anti yap1 pab, by Cell Signaling Technology (2 mentions)
Anti fibronectin pab, by Merck Group (2 mentions)
Anti arhgap18 pab, by Cell Signaling Technology (2 mentions)
Anti phospho ser1943 myh9 pab, by Cell Signaling Technology (2 mentions)
Anti myh10 mab, by Cell Signaling Technology (2 mentions)
Anti phospho mlc2 ser19, by Cell Signaling Technology (2 mentions)
Immobilon transfer membrane, by Merck Group (2 mentions)
2 protocols using anti myh9 pab
1
Spheroid Lysis and Protein Analysis
2
Spheroid Lysis and Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Spheroids were lysed in lysis buffer (0.5% TritonX-100, 150 mM NaCl, 20 mM Tris-HCl pH7.5). The lysates were sheared with a 21-gauge needle, incubated on ice for 30 minutes and clarified by centrifugation at 20,817 × g for 15 minutes at 4°C. The extracted proteins were separated by SDS-PAGE and transferred to immobilon transfer membrane (Millipore) for Western blotting analyses. The primary antibodies were anti-YAP1 pAb (#4912 Cell Signaling, 1:500), anti-Fibronectin pAb (F3648, Sigma Aldrich, 1:1000), anti-ARHGAP18 pAb (1:10000)17 (link),21 (link), anti-MYH9 pAb (#3403 Cell signaling, 1:1000), anti-Phospho Ser1943-MYH9 pAb (#5026 Cell Signaling, 1:1000), anti-MYH10 mAb (#8824 Cell Signaling, 1:1000), anti-Phospho-Ser19 MLC2 (#3675, Cell signaling, 1:100), and anti-GAPDH mAb (sc32233, Santa Cruz, 1:5000).
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