Phorbol-12-myristate-13-acetate (PMA), MSU, ATP, nigericin, poly A:T, Pam3CSK4, A9C, DIDS, and IAA94 were purchased from Sigma. MitoSox, MitoTracker Red, DAPI, MQAE, Lipofectamine RNAiMAX, Lipofectamine 2000, and M-MLV were from Invitrogen. Ultrapure LPS was obtained from Invivogen. SYRB Green was from TransGen Biotech. MnTBAP was from Santa Cruz. Salmonella is a gift from R.V. Bruggen. Anti-human cleaved IL-1β (2021, 1:1000 dilution) and anti-human caspase-1(2225, 1:1000 dilution) antibodies were purchased from Cell Signalling. Anti-human pro-IL-1β (60136-1-lg, 1:1000 dilution) anti-CLIC1(14545-1-AP, 1:500 dilution), anti-GAPDH (10494-1-AP, 1:1000 dilution), anti-VDAC1 (66345-1-lg, 1:1000 dilution), and anti-ATP1A1 (55187-1-AP, 1:1000 dilution) antibodies were from Proteintech. Anti-CLIC4 (SC-135739, 1:1000 dilution), anti-NEK7 (SC-50756, 1:500 dilution), and anti-ASC (rabbit, SC-22514, 1:400 dilution) antibodies were from Santa Cruz. Anti-ASC (mouse, 04–147, 1:1000 dilution) antibody was from Merck Millipore. Anti-CLIC5 (ACL-025, 1:250 dilution) antibody was from Alomone labs. Anti-mouse IL-1β (AF-401-NA, 1:1000 dilution) antibody was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042, 1:1000 dilution) and anti-NLRP3 (AG-20B-0014, 1:1000 dilution) antibodies were from Adipogen. Anti-β-actin (P30002, 1:1000 dilution) antibody was from Abmart.
Anti nek7 sc 50756
Manufactured by Santa Cruz Biotechnology
Anti-NEK7 (SC-50756) is an antibody product offered by Santa Cruz Biotechnology. It is a primary antibody that targets the NEK7 protein. NEK7 is a serine/threonine-protein kinase that plays a role in mitotic cell cycle progression. The antibody can be used for applications such as Western Blotting, Immunoprecipitation, and Immunohistochemistry, among others.
Automatically generated - may contain errors
Lab products found in correlation
Anti nlrp3 ag 20b 0014, by Adipogen (3 mentions)
Mitosox, by Thermo Fisher Scientific (3 mentions)
Adenosine triphosphate (atp), by Merck Group (3 mentions)
Nigericin, by Merck Group (3 mentions)
Anti mouse caspase 1 p20 ag 20b 0042, by Adipogen (3 mentions)
Poly a t, by Merck Group (2 mentions)
Protein g agarose, by Merck Group (2 mentions)
Pam3csk4, by Thermo Fisher Scientific (2 mentions)
Anti mouse il 1β p17 af 401 na, by R&D Systems (2 mentions)
Anti human caspase 1, by Cell Signaling Technology (2 mentions)
Ultrapure lps, by Thermo Fisher Scientific (2 mentions)
Streptavidin coated beads, by Merck Group (2 mentions)
Anti asc sc 22514 r, by Santa Cruz Biotechnology (2 mentions)
Anti β actin p30002, by Abmart (2 mentions)
Anti human cleaved il 1β a5208206, by Sangon (2 mentions)
Anti flag f2555, by Merck Group (2 mentions)
Mitotracker, by Thermo Fisher Scientific (2 mentions)
Phorbol 12, by Merck Group (1 mentions)
Anti vdac1, by Proteintech (1 mentions)
Ultrapure lps, by InvivoGen (1 mentions)
3 protocols using anti nek7 sc 50756
1
Cytokine Activation Pathway Analysis
2
NLRP3 Inflammasome Activation Pathway
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MSU, nigericin, ATP, PMA, BHB, DL-sulforaphane, poly(dA:dT), and glucose were purchased from Sigma-Aldrich. MCC950 was acquired from Selleck. BAY 11-7082, isoliquiritigenin, parthenolide, flufenamic acid, and mefenamic acid were acquired from Topscience. Human recombinant insulin was bought from Novo Nordisk. The One Touch Ultra Blood glucose Test System kit was bought from Roche. Ultrapure LPS, Pam3CSK4, MitoTracker, and MitoSOX were obtained from Invitrogen. Protein G agarose and streptavidin-coated beads were respectively supplied by Millipore and Pierce Biochemicals. ATP-coupled beads were from BioWorld Company. Anti-mouse caspase-1 (p20; AG-20B-0042) and anti-NLRP3 (AG-20B-0014) were from Adipogen. Anti-ASC (sc-22514-R) and anti-NEK7 (SC-50756) were from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17; AF-401-NA) was from R&D Systems. Anti-Flag (F2555) and anti-Mcherry (V4888) were from Sigma-Aldrich. Recombinant human NLRP3 was from Novus Biologicals. A standard HFD (D12492, 60% kcal fat) was from Research Diet Company.
3
NLRP3 Inflammasome Activation and IL-1β Regulation
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ATP, MSU, poly A:T, nigericin, and glucose were from Sigma. The human recombinant insulin was from Nova Nordisk. One Touch® Ultra® Blood glucose Test System was from Roche. Oridonin (s2335) was bought from Selleck. MitoSOX, ultrapure LPS, Pam3CSK4 and MitoTracker were from Invitrogen. Protein G agarose and streptavidin-coated beads were, respectively, supplied by Millipore and Pierce Biochemicals. Anti-Flag (F2555) and anti-VSV (V4888) were bought from Sigma. Anti-NLRP3 (AG-20B-0014) and anti-mouse caspase-1 (p20) (AG-20B-0042) antibodies were from Adipogen. Anti-NEK7 (SC-50756) and anti-ASC (sc-22514-R) antibodies were obtained from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D Systems. A standard high-fat diet (D12492, 60% kcal fat) was from Research Diet Company.
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