Annexin 5 fitc or annexin 5 apc apoptosis detection kit

Manufactured by BD

Sourced in United States

The Annexin V-FITC or Annexin V-APC Apoptosis Detection Kit is a laboratory instrument used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, which is externalized during the early stages of apoptosis. The Annexin V is conjugated with either fluorescein isothiocyanate (FITC) or allophycocyanin (APC) dyes, allowing for the detection and quantification of apoptotic cells through flow cytometry or fluorescence microscopy.

Automatically generated - may contain errors

Lab products found in correlation

Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

APC Annexin V Apoptosis Detection Kit FITC Annexin V Apoptosis Detection Kit Annexin V-FITC or APC FITC Annexin V Apoptosis Detection Annexin V-APC Apoptosis Kit Annexin V-FITC Apoptosis Kit Annexin V Apoptosis Detection Kit Annexin V-FITC detection kit Annexin V Apoptosis Kit APC Annexin V kit

2 protocols using annexin 5 fitc or annexin 5 apc apoptosis detection kit

Cell Apoptosis Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 1 × 10⁶ cells were resuspended in a single cell suspension and washed two times with PBS solution. The cell apoptosis analysis was performed with the Annexin V-FITC or Annexin V-APC Apoptosis Detection Kit (BD Biosciences, USA) according to the manufacturer’s instructions. The rates of apoptosis were detected by flow cytometry (BD FACSCanto II, USA).

Wu P., Cai J., Chen Q., Han B., Meng X., Li Y., Li Z., Wang R., Lin L., Duan C., Kang C, & Jiang C. (2019). Lnc-TALC promotes O6-methylguanine-DNA methyltransferase expression via regulating the c-Met pathway by competitively binding with miR-20b-3p. Nature Communications, 10, 2045.

+ Open protocol

+ Expand

Apoptosis Quantification by Flow Cytometry

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry analysis was performed as mentioned before^{9 (link)}. Briefly, For the cell apoptosis assay, A total of 1 × 10⁶ cells were resuspended in a single cell suspension and washed two times with PBS solution. The cell apoptosis analysis was performed with the Annexin V-FITC or Annexin V-APC Apoptosis Detection Kit (BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions. The rates of apoptosis were detected by flow cytometry (CyAn, Beckman Coulter).

Deng X., Kong F., Li S., Jiang H., Dong L., Xu X., Zhang X., Yuan H., Xu Y., Chu Y., Peng H, & Guan M. (2021). A KLF4/PiHL/EZH2/HMGA2 regulatory axis and its function in promoting oxaliplatin-resistance of colorectal cancer. Cell Death & Disease, 12(5), 485.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!