N methylmorpholine nmm

A functionalized carbon nonwoven (CF-1c) containing carboxyl groups on the surface was used to incorporate diamine derivative. The carbon nonwoven CF-1c was treated with a solution containing 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-morpholine (DMT/NMM/TosO⁻) [42 ] (0.21 g, 5 mmol) and N-methylmorpholine (NMM) (Sigma-Aldrich, Poznan, Poland) (550 µL, 5 mmol) in DMF (5 mL) as a solvent. The reaction of carboxyl group activation was carried out for 90 min at room temperature. Then the excess reactants were removed, and the nonwoven fabric was washed twice with DCM (5 mL) and treated with ethylenediamine (Sigma-Aldrich, Poznan, Poland) (0.45 mL, 7 mmol) in DCM. The condensation reaction was carried out for 12 h at room temperature. After this time, the nonwoven was washed with DMF (3 × 5 mL) and DCM (3 × 5 mL). The modification product was a nonwoven CF-C6H₄-CONH-(CH₂)₂-NH₂ (CF-2) containing free amine groups on the surface.

Fmoc-protected amino acids, 2-(6-Chloro-1H-benzotriazole-1-yl)-1, -1,3,3-tetramethylaminium hexafluorophosphate (HCTU), trifluoroacetic acid (TFA), and rink amide SS resin, were purchased from Advance ChemTech, Louisville, KY, USA. Nα-Fmoc-Nδ-allyloxycabonyl-L-ornithine (Fmoc-Orn[Aloc]-OH) was purchased from Chem-Impex International, Inc., Wood Dale, IL, USA. 1-Hydroxy-6-(trifluoromethyl) benzotriazole (HOBt) and (Ethyl cyano[hydroxyimino]acetato)-tri-(1-pyrrolidinyl)-phosphonium (PyBOP) were purchased from Novabiochem, Billerica, MA, USA. Dimethylformamide (DMF) was purchased from Protein Technologies, Tucson, AZ, USA. Diisopropylethylamine (DIEA), triisopropylsilane (TIPS), tetrakis(triphenylphosphine) palladium (0) (palladium), 5(6)-carboxyfluorescein (FAM), chloroform (CHCl₃), methanol (MeOH), dichloromethane (DCM), and N-methylmorpholine (NMM) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Glacial acetic acid was purchased from Alfa Aesar, Ward Hill, MA, USA. Acetic anhydride was purchased from Fisher Scientific, Fair Lawn, NJ, USA. Commercial peptide ARG (FAM-RRRRRRRRR) was purchased from AnaSpec, Fremont, CA, USA. All the salts used for the preparation of assay buffers were purchased from Sigma Aldrich, St. Louis, MO, USA.