Non-iners Lactobacillus isolates were maintained and quantitatively titered on Lactobacillus MRS Agar plates (purchased as prepared media from Hardy Diagnostics). Most other species, including L. iners, were maintained and titered on Columbia Blood Agar (“CBA”; purchased as prepared media from Hardy Diagnostics). Prevotella species were maintained on CBA agar or CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (“LKV”; purchased as prepared media from BD BBL™). For bacterial isolations from primary clinical samples, we used commercial CBA and LKV agar plates and prepared in-house MRS agar plates by autoclaving Lactobacillus MRS Broth (BD DIFCO™) in Noble Agar (BD DIFCO™, 2% w/v), cooling to 50°C with agitation, then supplementing with IsoVitaleX™ Enrichment (BD BBL™; 2% v/v) with or without Vitamin K1-Hemin Solution (BD BBL™; 5% v/v), or with freshly filter-sterilized stocks of L-Cys and L-Gln (at final concentrations of 4 mM and 1.1 mM respectively). All solid media was stored at 4°C in the dark after purchase or preparation until being introduced to the anaerobic chamber for deoxygenation and use.
Cdc anaerobe laked sheep blood agar
Manufactured by BD
CDC Anaerobe Laked Sheep Blood Agar is a culture medium used for the isolation and identification of anaerobic bacteria. It contains laked sheep blood, which provides essential nutrients and growth factors for anaerobic microorganisms.
Automatically generated - may contain errors
3 protocols using cdc anaerobe laked sheep blood agar
1
Anaerobic Bacterial Culture Protocol
2
Anaerobic Bacterial Culture Protocol
3
Anaerobic Bacterial Culture Media Preparation
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Non-iners Lactobacillus isolates were maintained and quantitatively titered on Lactobacillus MRS Agar plates (purchased as prepared media from Hardy Diagnostics). Most other species, including L. iners, were maintained and titered on Columbia Blood Agar ("CBA"; purchased as prepared media from Hardy Diagnostics). Prevotella species were maintained on CBA agar or CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin ("LKV"; purchased as prepared media from BD BBL™). For bacterial isolations from primary clinical samples, we used commercial CBA and LKV agar plates and prepared in-house MRS agar plates by autoclaving Lactobacillus MRS Broth (BD DIFCO™) in Noble Agar (BD DIFCO™, 2% w/v), cooling to 50°C with agitation, then supplementing with IsoVitaleX™ Enrichment (BD BBL™; 2% v/v) with or without Vitamin K1-Hemin Solution (BD BBL™; 5% v/v), or with freshly filter-sterilized stocks of L-Cys and L-Gln (at final concentrations of 4 mM and 1.1 mM respectively). All solid media was stored at 4°C in the dark after purchase or preparation until being introduced to the anaerobic chamber for deoxygenation and use.
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