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Luna silica 3 μm

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Luna silica 3 μm is a type of chromatography column packing material. It has a particle size of 3 micrometers and is composed of silica.

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Lab products found in correlation

1260 hplc, by Phenomenex (2 mentions) Uplc qtrap 6500 plus, by AB Sciex (1 mentions)

Close spelling variants of this product

Luna 3 μm Silica (2) 100 Å, Luna silica

3 protocols using luna silica 3 μm

1

Lipidomic Analysis of Cellular Lipids

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Cellular lipids were extracted using same method described for phosphoinositide analysis. Lipids were dried and diluted with 113 μL of chloroform:methanol:water (73:23:3) mixture and filtered (0.45 μm) before analysis on an Agilent 6230 electrospray ionization–time-of-flight (ESI-TOF) MS coupled to an Agilent 1260 HPLC equipped with a Phenomenex Luna silica 3 μm 100 Å 5 cm × 2.0 mm column. LCMS analysis was performed using normal phase HPLC with a binary gradient elution system where solvent A was chloroform:methanol:ammonium hydroxide (85:15:0.5) and solvent B was chloroform:methanol:water:ammonium hydroxide (60:34:5:0.5). Separation was achieved using a linear gradient from 100% A to 100% B over 9 min. Phospholipid species were detected using a dual ESI source operating in positive mode, acquiring in extended dynamic range from m/z 100–1700 at one spectrum per second; gas temperature: 325°C; drying gas 10 L/min; nebulizer: 20 psig; fragmentor 300 V
Wang D.G., Paddock M.N., Lundquist M.R., Sun J.Y., Mashadova O., Amadiume S., Bumpus T.W., Hodakoski C., Hopkins B.D., Fine M., Hill A., Yang T.J., Baskin J.M., Dow L.E, & Cantley L.C. (2019). PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Cell reports, 27(7), 1991-2001.e5.
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2

Sphingolipid Profiling of Ileum Tissues

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The collected ileum tissues were cryopreserved in liquid nitrogen, and five samples from each group were randomly selected, then 15 samples in three groups were collected under dry ice storage and sent to Changzhou Zhongke Zhidian Biotechnology Co., Ltd., China for sphingolipid group analysis. Lipids from the samples were extracted with an improved Bligh/Dyer extraction method (two extractions). The samples were reconstituted in the isotope mixture standard, and all analyses were performed in the Electrospray Ionization (ESI) mode using an Exion UPLC-QTRAP 6500 Plus (Sciex) LC/MS instrument [24 (link)]. Phenomenex Luna silica 3 μm (inner diameter 150×2.0 mm) chromatographic column was applied to separate various polar lipids under different conditions. Mass spectrometry multiple reaction monitoring (multiple IRactionmonitoring [MRM]) was established for the identification and quantitative analysis of various lipids [25 (link), 26 (link)]. The quantification of lipid substances is carried out by the added internal standard.
Li J., Zheng X., Li X., Yang J., Liu W., Yang L, & Liu B. (2022). Study on the protective effect and mechanism of Liriodendrin on radiation enteritis in mice. Journal of Radiation Research, 63(2), 213-220.
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3

Lipidomic Analysis of Cellular Lipids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular lipids were extracted using same method described for phosphoinositide analysis. Lipids were dried and diluted with 113 μL of chloroform:methanol:water (73:23:3) mixture and filtered (0.45 μm) before analysis on an Agilent 6230 electrospray ionization–time-of-flight (ESI-TOF) MS coupled to an Agilent 1260 HPLC equipped with a Phenomenex Luna silica 3 μm 100 Å 5 cm × 2.0 mm column. LCMS analysis was performed using normal phase HPLC with a binary gradient elution system where solvent A was chloroform:methanol:ammonium hydroxide (85:15:0.5) and solvent B was chloroform:methanol:water:ammonium hydroxide (60:34:5:0.5). Separation was achieved using a linear gradient from 100% A to 100% B over 9 min. Phospholipid species were detected using a dual ESI source operating in positive mode, acquiring in extended dynamic range from m/z 100–1700 at one spectrum per second; gas temperature: 325°C; drying gas 10 L/min; nebulizer: 20 psig; fragmentor 300 V
Wang D.G., Paddock M.N., Lundquist M.R., Sun J.Y., Mashadova O., Amadiume S., Bumpus T.W., Hodakoski C., Hopkins B.D., Fine M., Hill A., Yang T.J., Baskin J.M., Dow L.E, & Cantley L.C. (2019). PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Cell reports, 27(7), 1991-2001.e5.
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