PD-L1, CD8, and p16 (as a surrogate of HPV infection)20 (link),21 (link) were stained using a standard protocol as previously described.22 (link) Briefly, freshly cut formalin-fixed paraffin-embedded specimens were dewaxed in xylene, hydrated in graded alcohol, and washed in phosphate-buffered saline; after neutralizing endogenous peroxidase (0.3% H2O2 for 10 min), the microwave antigen retrieval method was applied using Tris–EDTA (pH 9.0). Subsequently, the slides were preincubated with blocking serum and then were incubated overnight at 4°C with each primary antibody: p16 (1:100, ZM0205; ZSGB-BIO, Beijing, People’s Republic of China), CD8 (1:100, ZA0508, ZSGB-BIO), and PD-L1 (1:100, #13684; Cell Signaling Technology, Shanghai, People’s Republic of China). Once the above procedure was completed, the sections were serially rinsed, incubated with secondary antibodies, visualized using diaminobenzidine, and counterstained with hematoxylin. Tonsil tissues were used as positive controls for PD-L1 and CD8, and cervical squamous cell carcinoma specimens were used as positive controls for p16 (Figure S1 ). Specimens of identical tissues stained without primary antibody were used as negative controls.
Zm 0205
Manufactured by ZSGB-BIO
Sourced in China
The ZM-0205 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable design, offering reliable performance and consistent results. The centrifuge can accommodate a range of sample volumes and tube sizes, making it suitable for a variety of laboratory workflows.
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Lab products found in correlation
3 protocols using zm 0205
1
Immunohistochemical Staining of PD-L1, CD8, and p16
2
Comprehensive Colposcopy and Biopsy Protocol
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The colposcopy procedure and biopsy were performed by experienced gynecological specialists from The Second Hospital of Shanxi Medical University. The average time from Pap sampling to colposcopy was 15 days (range: 3-62 days). Colposcopy examinations were performed using the Preventive Oncology International microbiopsy protocol. During the colposcopy, the cervix was divided into quadrants and each quadrant was examined independently. All visually abnormal areas were biopsied. Quadrants with normal colposcopic impressions were biopsied at the squamocolumnar junction (“random biopsy”). Women with abnormal cytology results and negative or inadequate colposcopic findings underwent endocervical curettage (ECC). All histological slides were reviewed by two gynecological pathologists, who were blinded to the cytology results at The Second Hospital of Shanxi Medical University. Immunohistochemistry for p16INK4A (p16) (ZM-0205, ZSGB-BIO, Peking, China) was utilized in adjudicating the diagnosis. If the two pathologists disagreed on the diagnosis, a third senior pathologist reviewed the slides, and some difficult or equivocal cases were considered for the pathology panel consensus diagnosis.
3
Immunohistochemical Analysis of CHFR and p16 in ESCC
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Twenty-eight ESCC samples was immunohistochemically stained by the Envision method. The sections (5 µm thick) were deparaffinized, rehydrated with graded concentrations of ethanol, and incubated with 3% H2O2 for 15 min. The sections were then incubated with the primary antibody (rabbit anti-human CHFR monoclonal antibodies, BS-4272R, Bioss, Beijing, China; mouse anti-human p16 monoclonal antibodies, ZM-0205, Zsbio, Beijing, China), followed by incubation with secondary antibodies (anti-mouse/rabbit, PV-6000, Zsbio, Beijing, China) for 15 min at room temperature and DAB reagent (ZLI-9018, Zsbio, Beijing, China).The sections were then counterstained with hematoxylin, dehydrated. CHFR, and CDKN2A (p16) staining were scored for nuclear and cytoplasmic staining based on intensity and percentage of cells stained (25 (link),26 (link)).
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