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2 protocols using potassium dihydrogen orthophosphate

1

Analytical Standards for Pharmaceutical Validation

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Primary analytical reference standards with purity ≥ 99% of ciprofloxacin (CPF), trimethoprim (TMP), sulphamethoxazole (SMZ), metronidazole (MTZ), amoxicillin (AMX), clavulanic acid (CLA), flucloxacillin (FCX), cefuroxime (CFX), arthemether (ART), lumefantrine (LUM), mebendazole (MBZ), albendazole (ALB), ferrous ammonium citrate (FAC), vitamin B1 (VB1), vitamin B3 (VB3), vitamin B6 (VB6), folic acid (FLA), griseofulvin (GFV), paracetamol (PCM), and ibuprofen (IBF) were obtained from the Food and Drugs Authority, Ghana. Analytical grade solvents including sodium hydroxide (BDH, UK), formic acid, methanol, chloroform acetic acid, glacial acetic acid (BDH, UK), ethanol, acetonitrile, ammonium hydroxide reagent ACS, sulphuric acid, ethyl acetate, holmium perchlorate, and potassium dihydrogen orthophosphate (BDH, UK) were used.
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2

Ethylphenidate and d3-Methylphenidate Analysis

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Certified standards for ethylphenidate and d3-methylphenidate were purchased from LGC Standards (Teddington, UK). Methanol, chloroform, isopropanol, hydrochloric acid, 35% ammonia solution and glacial acetic acid were purchased from Fisher Scientific (Loughborough, UK). Potassium dihydrogen orthophosphate was purchased from BDH (Poole, UK). Strata Screen-C solid phase extraction (SPE) cartridges were purchased from Phenomenex (Macclesfield, UK). Blank equine serum was purchased from TCS Biosciences (Buckingham, UK).
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