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Its g supplement

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ITS-G supplement is a defined, serum-free cell culture supplement. It provides insulin, transferrin, and selenium for the support of cell growth and proliferation in various cell culture applications.

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ITS-G (28 citations) ITS supplement (15 citations) Supplement G (2 citations)

2 protocols using its g supplement

1

Preparing Lipid-depleted Serum for Cell Culture

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To facilitate cell culture under defined lipid conditions, fetal bovine serum (FBS) devoid of sterols, triglycerides, and other neutral lipids was prepared based on previously described techniques (Cham and Knowles, 1976 (link)). To prevent oxidation due to trace peroxides, 0.1 mg ethylenediamine tetraacetate (EDTA) was added per 50 mL FBS. Serum was mixed 1:2 with organic phase (consisting of 3:2 ratio diisopropyl ether:n-butanol) followed by vigorous stirring for 1 h protected from light. After centrifugation at 4°C for 15 min at 2200 rpm, the aqueous layer was collected and lyophilized. Following resuspension in molecular grade H2O, ITS-G supplement (Invitrogen) was added, the solution filter-sterilized, and stored at −20°C. GC-MS analysis indicated nearly undetectable cholesterol levels. Lipoprotein-deficient, delipidated serum (LPDS) was substituted for regular FBS to stimulate expression of de novo cholesterol biosynthesis.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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2

Preparing Lipid-depleted Serum for Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
To facilitate cell culture under defined lipid conditions, fetal bovine serum (FBS) devoid of sterols, triglycerides, and other neutral lipids was prepared based on previously described techniques (Cham and Knowles, 1976 (link)). To prevent oxidation due to trace peroxides, 0.1 mg ethylenediamine tetraacetate (EDTA) was added per 50 mL FBS. Serum was mixed 1:2 with organic phase (consisting of 3:2 ratio diisopropyl ether:n-butanol) followed by vigorous stirring for 1 h protected from light. After centrifugation at 4°C for 15 min at 2200 rpm, the aqueous layer was collected and lyophilized. Following resuspension in molecular grade H2O, ITS-G supplement (Invitrogen) was added, the solution filter-sterilized, and stored at −20°C. GC-MS analysis indicated nearly undetectable cholesterol levels. Lipoprotein-deficient, delipidated serum (LPDS) was substituted for regular FBS to stimulate expression of de novo cholesterol biosynthesis.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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