The root and rhizome of Angelica sinensis (Oliv.) Diels and stem bark of Cinnamomum cassia (L.) J. Presl, were purchased from Guangzhou Zisun Pharmaceutical Co., Ltd., China, which are accordant with the standards of Chinese Pharmacopoeia (2015 edition) by confirmation of Professor Quan Zhu. We performed the method of steam distillation for the extracts of AC (1 : 1 of two herbal medicine weight ratio), and detailed procedures refer to our previous article [21 (link)]. The extracts (yield: 25%) were obtained by lyophilizing the concentrated sample with a Virtis Freeze Dryer (The Virtis Company, New York, USA). 5 mg of AC extract was dissolved in 5 ml of 30% methanol and filtered, which was directly subjected to ACQUITY UPLC system on PAD λe detector (λ = 200–400 nm), autosampler, in-line degasser, Waters ACQUITY-UPLC CLASS system (Waters Corp., Milford, USA) on an ACQUITY UPLC HSS T3 column (150 mm × 2.1 mm, 1.8 μm). Reference compounds are ferulic acid (t R = 2.59 min, purity HPLC > 98%), Senkyunolide I (t R = 4.42 min, purity HPLC > 98%), and Trans-Cinnamic acid (t R = 6.32 min, purity HPLC > 98%), which were purchased from Chengdu Chroma-Biotechnology Co., Ltd., China.
Waters acquity uplc class system
Manufactured by Waters Corporation
Sourced in United States
The Waters ACQUITY-UPLC CLASS system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features an ultra-high-pressure liquid chromatography (UPLC) module that enables rapid and efficient separation of complex samples. The system is capable of delivering precise and reproducible results, making it a reliable tool for various analytical workflows.
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Lab products found in correlation
Hss t3 column, by Waters Corporation (1 mentions)
Ferulic acid, by Chengdu Chroma-Biotechnology (1 mentions)
Senkyunolide 1, by Chengdu Chroma-Biotechnology (1 mentions)
Waters acquity uplc hss t3 column, by Waters Corporation (1 mentions)
Tq s micro triple quadrupole mass spectrometer, by Waters Corporation (1 mentions)
3 protocols using waters acquity uplc class system
1
Simultaneous UPLC Analysis of AC Extracts
2
Targeted Metabolite and Peptide Analysis
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Waters Xevo TQ-S Micro triple quadrupole mass spectrometer coupled with a Waters Acquity UPLC ® Class system was used to develop the MRM transitions of 20 identified metabolites and 4 intact GHRH synthetic analogs by direct infusion of standard solutions (10 µg/mL) using a post-column infusion mode. Once the MRM transitions were optimized, neat standard solutions were injected and the chromatographic separation was performed using a Waters Acquity UPLC ® HSS T3 column
3
Panax Notoginseng Saponins Extraction and Analysis
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Extraction of Panax Notoginseng and identi cation of ethanolic extract (PNE) and total saponins of Panax Notoginseng (PNS) Extraction of P. notoginseng was prepared as stated in our previous article [23] (link). In brief, the dried P. notoginseng powder was extracted with 95% ethanol and the ethanol was removed by rotary evaporation. Finally, the extract was freeze-dried to obtain PNE. PNS was obtained from Yunnan Yunke Pharmaceutical Co. Ltd (China). The component analysis of PNS and PNE was determined by Waters ACQUITY-UPLC CLASS system (Waters Corp., USA) with an ACQUITY UPLC BEH phenyl column (150 mm × 2.1 mm, 1.7 µm) maintained at 45°C to quantify the contents of notoginsenoside R1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, and ginsenoside Rd.
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