P tauser202 thr205

Manufactured by Thermo Fisher Scientific

P-tauSer202/Thr205 is a laboratory equipment product designed for the detection and quantification of phosphorylated tau proteins at serine 202 and threonine 205 residues. This product is intended to facilitate research and analysis related to the study of neurodegenerative diseases.

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Lab products found in correlation

Rat anti mouse b220 cd45r biotinylated, by BD (2 mentions) Neuronal nuclei (neun), by Vector Laboratories (2 mentions) Abc vector elite, by Vector Laboratories (2 mentions) Neuronal nuclei (neun), by Merck Group (2 mentions) Goat anti choline acetyltransferase chat, by Merck Group (2 mentions) Rabbit anti tyrosine hydroxylase th, by Merck Group (2 mentions) 3 3 diaminobenzidine (dab), by Vector Laboratories (2 mentions) Rat anti cd68, by Bio-Rad (2 mentions) Mouse anti at8, by Thermo Fisher Scientific (1 mentions) Mouse on mouse detection kit, by Vector Laboratories (1 mentions)

2 protocols using p tauser202 thr205

Immunostaining of Neuronal and Immune Markers

Cited 1 time

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For immunostaining, sections were immunostained according to standard protocols (Nguyen et al., 2014 (link)). The following antibodies were used: mouse anti-neuronal nuclei (NeuN; Millipore Sigma), goat anti-choline acetyl transferase (ChAT; Millipore Sigma), rabbit anti-tyrosine hydroxylase (TH; Millipore Sigma), mouse anti-AT8 [recognizes tau phosphorylated at serine 202 and threonine 205 (p-tau^{Ser202/Thr205}; ThermoFisher Scientific)], rat anti-CD68 (Bio-Rad AbD Serotec, Hercules, CA), rat anti-mouse B220/CD45R biotinylated (BD Biosciences, San Jose, CA), and hamster anti-CD3e (BD Biosciences). Briefly, free floating sections were immunolabeled with antibodies (1:500 for NeuN, 1:600 for ChAT, 1:1000 for TH, 1:800 for AT8, 1:1000 for CD68, 1:500 for B220/CD45R, and 1:1000 for CD3e) in conjunction with Mouse on Mouse Detection kit (for AT8), ABC Vector Elite, and 3,3′-diaminobenzidine (Vector Laboratories, Burlingame, CA) for visualization.

Nguyen T.V., Crumpacker R.H., Calderon K.E., Garcia F.G., Zbesko J.C., Frye J.B., Gonzalez S., Becktel D.A., Yang T., Tavera-Garcia M.A., Morrison H.W., Schnellmann R.G., Longo F.M, & Doyle K.P. (2022). Post-Stroke Administration of the p75 Neurotrophin Receptor Modulator, LM11A-31, Attenuates Chronic Changes in Brain Metabolism, Increases Neurotransmitter Levels, and Improves Recovery. The Journal of Pharmacology and Experimental Therapeutics, 380(2), 126-141.

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Immunostaining of Mouse Brain Sections

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For immunostaining, sections were immunostained according to standard protocols (Nguyen et al., 2014) . The following antibodies were used: mouse anti-neuronal nuclei (NeuN; Millipore Sigma), goat anti-choline acetyl transferase (ChAT; Millipore Sigma), rabbit anti-tyrosine hydroxylase (TH; Millipore Sigma), mouse anti-AT8 [recognizes tau phosphorylated at serine 202 and threonine 205 (p-tau Ser202/Thr205 ; ThermoFisher Scientific)], rat anti-CD68 (Bio-Rad AbD Serotec, Hercules, CA), rat anti-mouse B220/CD45R biotinylated (BD Biosciences, San Jose, CA), and hamster anti-CD3e (BD Biosciences). Briefly, free floating sections were immunolabeled with antibodies (1:500 for NeuN, 1:600 for ChAT, 1:1000 for TH, 1:800 for AT8, 1:1000 for CD68, 1:500 for B220/CD45R, and 1:1000 for CD3e) in conjunction with Mouse on Mouse (M.O.M.) Detection kit (for AT8), ABC Vector Elite, and 3,3'-diaminobenzidine (Vector Laboratories, Burlingame, CA) for visualization. (Franklin and Paxinos, 2008) was used as an anatomical reference atlas for mouse brain structures and Bregma coordinates.

Nguyen T.V., Crumpacker R.H., Calderon K., Garcia F.G., Zbesko J.C., Frye J.B., Gonzalez S., Becktel D.A., Yang T., Tavera-Garcia M.A., Morrison H.W., Schnellmann R.G., Longo F.M., & Doyle K.P. (2021). Post-stroke administration of the p75 neurotrophin receptor modulator, LM11A-31, attenuates chronic changes in brain metabolism, increases neurotransmitter levels, and improves recovery.

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