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6 phosphogluconate assay kit

Manufactured by Abcam
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The 6 Phosphogluconate Assay kit is a colorimetric assay designed to quantify the level of 6-phosphogluconate, an intermediate metabolite in the pentose phosphate pathway. The kit includes the necessary reagents and a protocol to perform the assay.

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Lab products found in correlation

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2 protocols using 6 phosphogluconate assay kit

1

Quantification of Metabolites in Kidney AKI

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The amount of pyruvate was measured using Pyruvate Assay Kit (Sigma). Kidneys harvested from Akr1a1+/+, Akr1a1−/−, Pkm2+/+ or Pkm2−/− mice (sham operation or AKI) were mechanically homogenized in Pyruvate Assay Buffer (1 mg/5 μl buffer). After extracts were clarified by centrifugation (20,000g, 4 °C, 20 min, ×2), supernatant was used for assay. GHB in the serum of Akr1a1+/+ and Akr1a1−/− mice was measured following the GHB enzymatic assay kit from BUHLMANN. For measuring PEP, 6PG, ATP, ADP and serine in HEK cells, 1 × 106 cells were lysed in corresponding buffer. The amount of PEP, 6PG, ATP, ADP and serine were respectively measured using PEP Colorimetric/Fluorometric Assay Kit (Sigma), 6 Phosphogluconate Assay kit (abcam), ATP Colorimetric/Fluorometric Assay Kit (Sigma), ADP Colorimetric/Fluorometric Assay Kit (Sigma) and DL-Serine Assay kit (Fluorometric) (Biovision).
Zhou H.L., Zhang R., Anand P., Stomberski C.T., Qian Z., Hausladen A., Wang L., Rhee E.P., Parikh S.M., Karumanchi S.A, & Stamler J.S. (2018). Metabolic reprogramming by the S-nitroso-CoA Reductase system protects from kidney injury. Nature, 565(7737), 96-100.
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2

Quantification of Metabolites in Kidney AKI

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amount of pyruvate was measured using Pyruvate Assay Kit (Sigma). Kidneys harvested from Akr1a1+/+, Akr1a1−/−, Pkm2+/+ or Pkm2−/− mice (sham operation or AKI) were mechanically homogenized in Pyruvate Assay Buffer (1 mg/5 μl buffer). After extracts were clarified by centrifugation (20,000g, 4 °C, 20 min, ×2), supernatant was used for assay. GHB in the serum of Akr1a1+/+ and Akr1a1−/− mice was measured following the GHB enzymatic assay kit from BUHLMANN. For measuring PEP, 6PG, ATP, ADP and serine in HEK cells, 1 × 106 cells were lysed in corresponding buffer. The amount of PEP, 6PG, ATP, ADP and serine were respectively measured using PEP Colorimetric/Fluorometric Assay Kit (Sigma), 6 Phosphogluconate Assay kit (abcam), ATP Colorimetric/Fluorometric Assay Kit (Sigma), ADP Colorimetric/Fluorometric Assay Kit (Sigma) and DL-Serine Assay kit (Fluorometric) (Biovision).
Zhou H.L., Zhang R., Anand P., Stomberski C.T., Qian Z., Hausladen A., Wang L., Rhee E.P., Parikh S.M., Karumanchi S.A, & Stamler J.S. (2018). Metabolic reprogramming by the S-nitroso-CoA Reductase system protects from kidney injury. Nature, 565(7737), 96-100.
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