Airyscan super resolution confocal microscope

Fluorescence was visualized and captured using a ZEISS LSM800 with Airyscan Super-resolution confocal microscope (Carl Zeiss Microscopy, Germany). Images were obtained using uniform gain settings with excitation wavelengths of 488 nm (green; IBA-1 staining), 561 nm (red; TUNEL staining) and 358 nm (bisbenzimide), and processed using ZEISS Zen (blue edition) software.

Fluorescence was visualised and captured by using a ZEISS LSM800 with Airyscan Super-resolution confocal microscope (ZEISS Microscopy, BW, Germany). Excitation wavelengths of 488 nm (green), 561 nm (red) and 358 nm (Hoechst) were used for image acquisition. Images were obtained using uniform gain settings and processed using the ZEISS Zen (blue edition) software and ImageJ (NIH, MA, US). H&E staining was captured using the brightfield setting of a laser-scanning A1 confocal microscope (Nikon Instruments, Inc., Tokyo, Japan). High-magnification images of retinal histology were taken as representative images from the superior retina at 500 μm from the optic nerve head as reported previously [52 (link)].