Rat anti ciap2

Western blot analysis was performed as described previously [25 (link)] using the following antibodies: mouse anti-XIAP, mouse anti-RIP1, mouse anti-FADD from BD Biosciences (Heidelberg, Germany), goat anti-cIAP1 from R&D Systems (Wiesbaden, Germany), rat anti-cIAP2, mouse anti-caspase 8 from Enzo Life Sciences (Lörrach, Germany), rabbit anti-caspase-3 from Cell Signaling Technology (Danvers, USA), rabbit anti-caspase 8 from Epitomics (Burlingame, USA) and mouse anti-GAPDH from HyTest (Turku, Finnland) followed by goat-anti-mouse IgG or goat-anti-rabbit IgG conjugated to horseradish peroxidase purchased from Santa Cruz Biotechnology, Inc. (Dallas, USA). Enhanced chemiluminescence was used for detection from Amersham Bioscience (Freiburg, Germany). Further, donkey anti-mouse IgG, donkey anti-rabbit IgG or donkey anti-goat IgG labeled with IRDye infrared dyes were used for fluorescence detection at 700 nm 800 nm (LI-COR Biotechnology, Bad Homburg, Germany). Immunoprecipitation was performed as previously described [26 (link)] using RIPA buffer or NP-40 buffer for cell lysis.

Western blot analysis was performed as described previously [44 (link)] using the following antibodies: Mouse anti-cFLIP (Enzo Life Sciences, Lörrach, Germany), goat anti-cIAP1 (R&D Systems, Wiesbaden, Germany), rat anti-cIAP2 (Enzo Life Sciences), mouse anti-XIAP (BD Biosciences, Heidelberg, Germany), rabbit anti-NIK (Cell Signaling Technologies, Beverly, MA USA), mouse anti-p100/p52 (Merck Millipore, Darmstadt, Germany), rabbit anti-p62 (MBL International, Woburn, MA, USA), mouse anti-Noxa (Enzo Life Sciences), rabbit anti-Mcl-1 (Enzo Life Sciences), mouse anti-caspase-8 (Enzo Life Sciences), rabbit anti-caspase-3 (Cell Signaling Technologies), rabbit anti-caspase-9 (Cell Signaling Technologies), mouse anti-β-actin (Sigma-Aldrich), mouse anti-GAPDH (HyTest, Turku, Finland), mouse anti-tubulin (Calbiochem, Merck Millipore, Darmstadt, Germany). Secondary antibodies conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used for enhanced chemiluminescence detection (Amersham Bioscience, Freiburg, Germany). Alternatively, secondary antibodies labeled with IRDye infrared dyes were used for fluorescence detection (Odyssey Imaging System, LI-COR Bioscience, Bad Homburg, Germany). All Western blots shown are representative of two or three independent experiments.