Immpress alkaline phosphatase kit

The follicles were fixed in 4% formaldehyde, embedded in paraffin, and sectioned at 7 μm. Immunostaining was performed in the Markey Biospecimen Procurement and Translational Pathology Shared Resource Facility at the University of Kentucky, as previously described (28 (link)). Briefly, heat-induced epitope retrieval was performed in a Biocare Medical Decloaking chamber using Dako's low pH Target Retrieval Solution. Primary antibody incubation was performed at 4^oC overnight for ACE2 (MilliporeSigma, HPA000288, 1:200 dilution) and for 2 hours at room temperature for platelet and endothelial cell adhesion molecule 1 (PECAM1) (Roche Diagnostics, Nederlands JC70 monoclonal antibody, predilute), respectively. Rabbit IgG was used in place of primary antibodies as a negative control. Antibody staining was detected with an appropriate Immpress alkaline phosphatase kit and Vector Red AP chromogen (Vector Laboratories, Burlingame, CA).