Agilent hplc chip

The collected cut-out piece from the silver-stained gel was trypsinized in a trypsin digestion buffer (10 mM CaCl₂, 100 mM ammonium bicarbonate, pH 7.8) overnight at 37 °C. The digested sample was subjected to protein identification using a nano-flow liquid chromatography-mass spectrometry apparatus (Agilent 6330 Ion Trap; Agilent Technologies, Santa Clara, CA) equipped with an analytical chip (Agilent HPLC-Chip; Agilent Technologies). The resulting tandem mass spectrometry spectra of the tryptic peptides were analyzed using Agilent software (Spectrum Mill MS Proteomics Workbench; Agilent Technologies) with the protein database (SwissProt) for putative Homo sapiens protein identifications.

The CBB-stained bands, BP1, 2, and 3, were cut out from the gel, and their individual pieces were individually trypsinized in a trypsin digestion buffer (10 mM CaCl2, 100 mM ammonium bicarbonate, pH 7.8) overnight at 37 °C. The digested liquid specimens were then subjected to a protein identification procedure using a nano-flow liquid chromatography–mass spectrometry apparatus (Agilent 6330 Ion Trap; Agilent Technologies, Santa Clara, CA, USA) equipped with an analytical chip (Agilent HPLC-Chip; Agilent Technologies). The resulting tandem mass spectrometry spectra of the tryptic peptides were analyzed using Agilent software (Spectrum Mill MS Proteomics Workbench; Agilent Technologies) with a protein database (SWISS-PROT).