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Fluoview confocal microscope

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The FluoView confocal microscope is a laboratory equipment designed for high-resolution imaging of biological samples. It utilizes laser technology to capture detailed, three-dimensional images of cells and tissues. The FluoView system allows for the observation of fluorescently labeled specimens with exceptional clarity and precision.

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Lab products found in correlation

Ab184601, by Abcam (2 mentions) Fitc conjugated anti mouse secondary antibody, by Vector Laboratories (2 mentions) Cy3 red conjugated anti rabbit secondary antibody, by Vector Laboratories (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)

2 protocols using fluoview confocal microscope

1

Quantifying Tumor Cell Populations Using Immunofluorescence

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Frozen xenograft tumor sections (5 um) were subjected to double-immunofluorescence staining by simultaneous incubation of sections with mouse anti-EGFP antibody (Abcam Cat. Ab184601) and rabbit anti-αSMA antibody (Abcam, Cat. 5694) overnight at 4℃ and then incubated with FITC-conjugated anti-mouse secondary antibody and Cy3 red-conjugated anti-rabbit secondary antibody (Vector Laboratories) for 1 hour at room temperature. Slides were counterstained with 2 mg/ml DAPI (Vector Laboratories). Specimens were observed with an Olympus FluoView confocal microscope, and images were analyzed with Adobe Photoshop. The numbers of DAPI+ cells, EGFP+ cells and /or α-SMA+ were counted under × 400 magnification in five random chosen fields. BMFs fluoresced yellow because of the overlapping green and red emissions. Percentage of positive cells was expressed as an average of the ratios of positive staining cells to the total DAPI positive number in 5 random at 400 magnification from tumor sections of three mice (n=15).
Zhu L., Cheng X., Shi J., Jiacheng L., Chen G., Jin H., Liu A.B., Pyo H., Ye J., Zhu Y., Wang H., Chen H., Fang J., Cai L., Wang T.C., Yang C.S, & Tu S.P. (2016). Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene, 35(41), 5388-5399.
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2

Quantifying Tumor Cell Populations Using Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen xenograft tumor sections (5 um) were subjected to double-immunofluorescence staining by simultaneous incubation of sections with mouse anti-EGFP antibody (Abcam Cat. Ab184601) and rabbit anti-αSMA antibody (Abcam, Cat. 5694) overnight at 4℃ and then incubated with FITC-conjugated anti-mouse secondary antibody and Cy3 red-conjugated anti-rabbit secondary antibody (Vector Laboratories) for 1 hour at room temperature. Slides were counterstained with 2 mg/ml DAPI (Vector Laboratories). Specimens were observed with an Olympus FluoView confocal microscope, and images were analyzed with Adobe Photoshop. The numbers of DAPI+ cells, EGFP+ cells and /or α-SMA+ were counted under × 400 magnification in five random chosen fields. BMFs fluoresced yellow because of the overlapping green and red emissions. Percentage of positive cells was expressed as an average of the ratios of positive staining cells to the total DAPI positive number in 5 random at 400 magnification from tumor sections of three mice (n=15).
Zhu L., Cheng X., Shi J., Jiacheng L., Chen G., Jin H., Liu A.B., Pyo H., Ye J., Zhu Y., Wang H., Chen H., Fang J., Cai L., Wang T.C., Yang C.S, & Tu S.P. (2016). Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene, 35(41), 5388-5399.
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