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8 oh dg elisa kit

Manufactured by Cayman Chemical
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Sourced in United States

The 8-OH-dG ELISA kit is a laboratory product designed to quantify the levels of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in biological samples. 8-OH-dG is a biomarker for oxidative DNA damage. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) format to detect and measure 8-OH-dG concentrations.

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ELISAs (3 citations)

2 protocols using 8 oh dg elisa kit

1

Assessing Placental and Fetal Oxidative Damage

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The levels of 8-hydroxydeoxyguanosine (8-OHdG) were assessed in the placental and fetal DNA to evaluate the oxidative damage produced by the UFP. The DNAzol reagent (Molecular Research Center, Cincinnati, OH, USA) was used to isolate genomic DNA of whole placentas and fetuses (as previously described). An 8-OH-dG ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) was used, and all procedures were conducted according to the manufacturer’s instructions.
Morales-Rubio R.A., Alvarado-Cruz I., Manzano-León N., Andrade-Oliva M.D., Uribe-Ramirez M., Quintanilla-Vega B., Osornio-Vargas Á, & De Vizcaya-Ruiz A. (2019). In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice. Particle and Fibre Toxicology, 16, 7.
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2

Quantifying 8-OHdG DNA Damage

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Competitive ELISA was performed using an 8-OHdG ELISA kit, by the protocol of the Cayman Chemicals (USA). The purified DNA was subjected to enzymatic digestion using nuclease P1 at 50 °C for 1 h and with alkaline phosphatase at 37 °C for 30 minutes. The digested DNA was boiled for 10 minuets and placed on ice for 5 minutes. The hydrolyzed DNA was measured by reading the absorbance at 412 nm. The levels of 8-OHdG were 78 measured and expressed as µg of 8-OHdG/ml.
KGK D., Kumari S., & Malla R.R. (2021). Marine Cyclic Dipeptide Cyclo (L-Leu-L-Pro) Protects Normal Breast Epithelial Cells from tBHP-induced Oxidative Damage by Targeting CD151. Archives of Breast Cancer.
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