At least three different mammary tumors from seven distinct PDXs were collected, digested into a single-cell suspension as described previously [16 , 17 (link)] and then plated in M87 medium. Saracatinib, bosutinib, and dasatinib were purchased from ApexBio and used at 10 μM for 72-h cytotoxicity assays. Cell viability was quantified using luciferase-based imaging, and viability of drug-treated cells was compared to that of vehicle-treated cells. All assays were performed as at least three biological replicates in triplicate.
Saracatinib
Manufactured by Apexbio
Sourced in United States
Saracatinib is a tyrosine kinase inhibitor. It is a laboratory reagent used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dasatinib, by Apexbio (1 mentions)
Oxaliplatin, by Apexbio (1 mentions)
P lrp6, by Proteintech (1 mentions)
Axin2, by Abcam (1 mentions)
Abcg1, by Proteintech (1 mentions)
Axin2, by Proteintech (1 mentions)
Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions)
Vimentin, by Abcam (1 mentions)
E cadherin, by Abcam (1 mentions)
β catenin, by Abcam (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Gsk3β, by Proteintech (1 mentions)
Bcl 2, by Proteintech (1 mentions)
2 protocols using saracatinib
1
Evaluating Mammary Tumor Responses to Tyrosine Kinase Inhibitors
2
Establishing Drug-Resistant Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Oxaliplatin, and Src inhibitor saracatinib (AZD0530) were used for the construction of drug-resistant cell lines, and other anti-cancer molecular targeting drugs were purchased from ApexBio (Houston, TX, USA) and Selleck (Houston, TX, USA). Monoclonal antibodies to the following proteins were used in western blot: E-cadherin, vimentin, PCNA, FZD8, DKK1, AXIN2, WNT6, and β-catenin (purchased from Abcam, Cambridge, MA, USA) and p-LRP6, GSK-3β, AXIN2, cyclin D1, SRC, OCT4, ABCG1, and BCL-2 (purchased from Proteintech, Chicago, IL, USA).
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