Methods are similar to those reported previously36 (link). Following a midline abdominal incision, the bladder was cannulated through the dome with a modified 14G BD Angiocath catheter connected to PE 90 tubing introduced with a hypodermic needle, secured with a purse string suture (4-0 silk, Cat. No M-S418R19, AD Surgical, Sunnyvale, CA), and connected to a solid-state pressure transducer (Deltran, Utah Medical, UT) to measure bladder pressure. A force transducer (model: MLT500D, AD Instruments, Colorado Springs, CO) was used to collect voided volume (VV). The external anal sphincter (EAS) EMG activity was measured by PFA-coated platinum-iridium wires (0.0055 inch-diameter, A-M Systems, Sequim, WA) inserted percutaneously into the EAS bilaterally. EAS EMG leads were connected through a preamplifier (HIP5, Grass Products, Warwick, RI) to an amplifier (P511, Grass Products). Bladder pressure (BP), VV, and EAS EMG signals were amplified, filtered, and sampled at either 1000 Hz (BP and VV) or 20,000 Hz (EAS EMG).
Mlt500d
Manufactured by ADInstruments
The MLT500D is a high-performance animal study transducer that measures changes in pressure or force. It is designed for use in various research applications, including cardiovascular, respiratory, and gastrointestinal studies. The device features a robust design and provides accurate and reliable data acquisition.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mlt500d
1
Bladder Pressure and Voiding Dynamics
2
Tibialis Anterior Muscle Tonic Contraction Force
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The tibialis anterior muscle was separated, the adjacent joints were fixed on a special fixation frame, and the distal tendons were cross-sutured with silk threads and connected to the traction receptors (MLT500/D, Force Transducer, ADInstruments) so that the direction of the muscle and the receptors was maintained in the same straight line. The traction tension was adjusted to maintain a certain degree of initial load (0 < F < 0.1 N). A stimulating electrode was placed on the sciatic nerve, and continuous electrical stimulation was given at 0.9 mA, a wave width of 0.1 ms, and a frequency of 50 Hz. The waveform of the tonic contraction force was recorded.
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