Incb024360 incb

Manufactured by Bio-Techne

Sourced in United Kingdom

INCB024360 (INCB) is a laboratory product offered by Bio-Techne. It is a chemical compound that functions as an inhibitor. The core function of INCB024360 is to inhibit specific biological targets, but a detailed description of its intended use is not available in an unbiased and factual manner while maintaining conciseness.

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Lab products found in correlation

1 methyl d tryptophan mt, by Merck Group (4 mentions) Penicillin streptomycin, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Htb 4, by American Type Culture Collection (4 mentions) Rpmi 1640 medium, by Vitrocell (4 mentions) Htb 2, by American Type Culture Collection (4 mentions)

4 protocols using incb024360 incb

Investigating IDO Inhibitors on Bladder Cancer Cells

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Human bladder cancer T24 cells and RT4 cells (HTB-4 and HTB-2, respectively; American Type Culture Collection-ATCC, Manassas, VA, USA) were acquired from the Cell Bank of the Federal University of Rio de Janeiro. T24 cells were cultured in RPMI 1640 Medium (Vitrocell, Campinas, Brazil) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO) and maintained at 37 °C with 5% CO₂.
To analyze the effect of IDO inhibitors on the expression of AHR and CYP1A1, RT4 and T24 cells were incubated with 1 μM INCB024360 (INCB, Tocris Bioscience, Bristol, UK) or 1 mM 1-methyl-D-tryptophan (MT, Sigma-Aldrich, St. Louis, MO) for 48 h. INCB was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO) for a 1 mM stock solution. MT was dissolved in 0.1 N NaOH for a 20 mM stock solution and then neutralized to pH 7.4 with 0.1 N HCl.
Cells were incubated into 6-well plates until 80% confluence. At this time, the medium was removed and medium containing IDO inhibitors was added. After 48 h, the cells were harvested and pelleted, being frozen at nitrogen and kept at − 80 °C for storage until RNA extraction. Supernatant was collected and maintained at − 80 °C for kynurenine measurement. The experiments were carried out in triplicates and repeated three times at different times.

Matheus L.H., Dalmazzo S.V., Brito R.B., Pereira L.A., de Almeida R.J., Camacho C.P, & Dellê H. (2020). 1-Methyl-D-tryptophan activates aryl hydrocarbon receptor, a pathway associated with bladder cancer progression. BMC Cancer, 20, 869.

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Evaluating IDO Inhibitors on AHR and CYP1A1

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Human bladder cancer T24 cells and RT4 cells (HTB-4 and HTB-2, respectively; American Type Culture Collection-ATCC, Manassas, VA, USA) were acquired from the Cell Bank of the Federal University of Rio de Janeiro. T24 cells were cultured in RPMI 1640 Medium (Vitrocell, Campinas, Brazil) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO) and maintained at 37°C with 5% CO 2 .
To analyze the effect of IDO inhibitors on the expression of AHR and CYP1A1, RT4 and T24 cells were incubated with 1 µM INCB024360 (INCB, Tocris Bioscience, Bristol, UK) or 1 mM 1-methyl-D-tryptophan (MT, Sigma-Aldrich, St. Louis, MO) for 48 hours. INCB was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO) for a 1 mM stock solution. MT was dissolved in 0.1 N NaOH for a 20 mM stock solution and then neutralized to pH 7.4 with 0.1 N HCl.
Cells were incubated into 6-well plates until 80 % con uence. At this time, the medium was removed and medium containing IDO inhibitors was added. After 48 hours, the cells were harvested and pelleted, being frozen at nitrogen and kept at -80° C for storage until RNA extraction. Supernatant was collected and maintained at -80° C for kynurenine measurement. The experiments were carried out in triplicates and repeated three times at different times.

Dellê H., Matheus L.H.G., Dalmazzo S.V., Brito R.B.O., Pereira L.A., Almeida R.J.d., & Camacho C. (2020). 1-Methyl-D-tryptophan activates aryl hydrocarbon receptor, a pathway associated with bladder cancer progression.

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IDO Inhibitors Modulate AHR and CYP1A1 in Bladder Cancer

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Human bladder cancer T24 cells and RT4 cells (HTB-4 and HTB-2, respectively; American Type Culture Collection-ATCC, Manassas, VA, USA) were acquired from the Cell Bank of the Federal University of Rio de Janeiro. T24 cells were cultured in RPMI 1640 Medium (Vitrocell, Campinas, Brazil) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO) and maintained at 37 °C with 5% CO 2 .
To analyze the effect of IDO inhibitors on the expression of AHR and CYP1A1, RT4 and T24 cells were incubated with 1 µM INCB024360 (INCB, Tocris Bioscience, Bristol, UK) or 1 mM 1-methyl-D-tryptophan (MT, Sigma-Aldrich, St. Louis, MO) for 48 hours. INCB was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO) for a 1 mM stock solution. MT was dissolved in 0.1 N NaOH for a 20 mM stock solution and then neutralized to ph 7.4 with 0.1 N HCl.
Cells were incubated in 6-well plates at 80% con uence. At this time, the medium was refreshed by containing IDO inhibitors was added. After 48 hours, the cells were pelleted and frozen at nitrogen and then at -80° C for RNA extraction. Supernatant was collected and maintained at -80° C for kynurenine measurement. The experiments were carried out in triplicate and repeated three times at different times.

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Evaluating IDO Inhibitors on AHR and CYP1A1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bladder cancer T24 cells and RT4 cells (HTB-4 and HTB-2, respectively; American Type Culture Collection-ATCC, Manassas, VA, USA) were acquired from the Cell Bank of the Federal University of Rio de Janeiro. T24 cells were cultured in RPMI 1640 Medium (Vitrocell, Campinas, Brazil) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO) and maintained at 37°C with 5% CO 2 .
To analyze the effect of IDO inhibitors on the expression of AHR and CYP1A1, RT4 and T24 cells were incubated with 1 µM INCB024360 (INCB, Tocris Bioscience, Bristol, UK) or 1 mM 1-methyl-D-tryptophan (MT, Sigma-Aldrich, St. Louis, MO) for 48 hours. INCB was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO) for a 1 mM stock solution. MT was dissolved in 0.1 N NaOH for a 20 mM stock solution and then neutralized to pH 7.4 with 0.1 N HCl.
Cells were incubated into 6-well plates until 80 % con uence. At this time, the medium was removed and medium containing IDO inhibitors was added. After 48 hours, the cells were harvested and pelleted, being frozen at nitrogen and kept at -80° C for storage until RNA extraction. Supernatant was collected and maintained at -80° C for kynurenine measurement. The experiments were carried out in triplicates and repeated three times at different times.

Matheus L.H.G., Dalmazzo S.V., Brito R.B.O., Pereira L.A., Almeida R.J.d., Camacho C., & Dellê H. (2020). 1-Methyl-D-tryptophan activates aryl hydrocarbon receptor, a pathway associated with bladder cancer progression.

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