Several human cancer cell lines were used in this study, including renal clear adenocarcinoma 786-O, pancreatic adenocarcinoma Panc-1, Burkitt’s lymphoma Raji, and ovarian cancer SKOV-3. These cells were purchased from PUMC National Cell Resource Corporation (Peking Union Medical College, Beijing, China) and cultured in RPMI-1640 medium (Gibco, Invitrogen) supplemented with 10% heat‐inactivated Fetal Bovine Serum (FBS; Gibco) and 1% penicillin-streptomycin at 37°C with 5% CO2. All cell lines were cultured for less than 6 passages before experiments. The host strains Escherichia coli (E. coli) DH5α and BL21 (DE3) were purchased from New England Biolabs (Neb, Beijing branch) and were used for cloning and expressing recombinant proteins, respectively.
Escherichia coli e coli dh5α
Manufactured by New England Biolabs
Escherichia coli (E. coli) DH5α is a laboratory strain of the bacterium E. coli. It is commonly used as a host for cloning and plasmid amplification in molecular biology experiments.
Automatically generated - may contain errors
Lab products found in correlation
Bl21 de3, by New England Biolabs (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sudan red 7b, by Merck Group (1 mentions)
N decane, by Merck Group (1 mentions)
β galactosidase from aspergillus oryzae, by Merck Group (1 mentions)
Zonyl fs 300, by ABCR (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
2 protocols using escherichia coli e coli dh5α
1
Culturing Cell Lines and Bacterial Hosts
2
Emulsion Formulation and Characterization
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The following materials were used as received without further purification: n-Decane (Sigma Aldrich, 99%), methoxyperfluorobutane (ABCR, 97%), Sudan Red 7B (Sigma Aldrich), di-n-octyl sulfosuccinate sodium salt (Sigma Aldrich, 98%), Zonyl FS-300 (ABCR), sodium dodecyl sulfate (Sigma Aldrich, 98%), β-n-octyl-galactopyranoside (Combi-Blocks), Escherichia coli (E.coli DH5 α- New England Biolabs), β-Galactosidase from Aspergillus oryzae (Sigma-Aldrich). Bacteria experiments were performed in PBS buffer solution (pH: 7.2). DI water was used for the emulsion continuous phase preparation.
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