Formalin fixed, paraffin-embedded intestinal (cecal, ileum) tissue sections (4 μm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens (1) histamine receptor 2 (AHR-002, Alomone labs, Israel), (2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer’s instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histofine simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa fluor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by hematoxylin, or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo Fisher, USA) as previously described for visualization of cell nuclei.
Ahr 002
Manufactured by Alomone
Sourced in Israel
AHR-002 is a product designed for laboratory use. It serves as a research tool for scientific investigations. The core function of AHR-002 is to facilitate controlled experiments and data collection. No further details are provided to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab2378, by Abcam (4 mentions)
Diaminobenzidine substrate kit, by Nacalai Tesque (4 mentions)
Alexa uor 647, by Abcam (3 mentions)
6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions)
Ab124732, by Abcam (1 mentions)
Ab110413, by Abcam (1 mentions)
Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions)
Chemidoc imager, by Bio-Rad (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
5 protocols using ahr 002
1
Immunohistochemical Characterization of Intestinal Mast Cells
2
Histochemical Analysis of Kidney Proteins
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Kidney tissues were cut into 1-to 2-mm slices, and the pieces were homogenized using a bead mill homogenizer at 5 m/s for 30 s to 1 min in RIPA buffer containing a protease inhibitor cocktail (Roche) on ice and then spin cleared at 10,000 g for 10 min. The resulting supernatant was subjected to SDS-PAGE and transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) for probing with primary and secondary antibodies, and the bands were subsequently visualized by enhanced chemiluminescence (Thermo Scientific, Waltham, MA) using a Chemidoc Imager (Bio-Rad). The following antibodies were used: HDC (PA5-79354, Invitrogen, 1:1,000), HNMT (PA5-57289, Invitrogen, 1:500), DAO (no. 13273-1-AP, Protein Tech, 1:400), HR1 (no. 13413-1-AP, Protein Tech, 1:500), HR2 (AHR-002, Alomone, 1:500), HR3 (ab124732, Abcam, 1:1,000), HR4 (bs-10993R, Bioss, 1:1,000), tryptase (ab110413, Abcam, 1:1,000), arginine vasopressin receptor 2 (AVPR2; PA5-68479, Invitrogen, 1:600), aquaporin-2 (AQP2; PA5-78808, Invitrogen, 1:250), and Fc fragment of IgE, high affinity I, receptor for α-polypeptide (FCER1A; A1751, ABClonal, 1:750).
3
Immunohistochemical Analysis of Intestinal Tissue
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Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
4
Immunohistochemical Analysis of Intestinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
5
Immunohistochemical Analysis of Intestinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin xed, para n-embedded intestinal (cecal, ileum) tissue sections (4 µm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens 1) histamine receptor 2 (AHR-002, Alomone labs, Israel), 2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer's instructions. Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Histo ne simple stain anti-rabbit, 414341F, Nacalai, USA and Alexa uor® 647, ab150131, Abcam, USA). Sections were counter-stained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by haematoxylin or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo scher, USA) as previously described for visualization of cell nuclei.
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