TLC aluminium sheets 20 × 20 cm silica gel 60 F254, silica gel 60 (Merck, Darmstadt, Germany), and pre-coated TLC glass plates SIL G-25 UV254, 0.25 mm silica gel (Sigma, St. Louis, MO, USA) were used for thin layer chromatography analysis, vacuum liquid column chromatography, and preparative TLC, respectively. Spots on TLC were visualized by using spraying reagent of Liebermann Burchard for terpenoid/steroid detection. Shimadzu QP-2010 Gas Chromatograph Mass Spectrometer Ultra (Shimadzu, Kyoto, Japan) was used for GC-MS analysis. Acquity UPLC I-Class System with the XEVO G2-XS QTof Mass Spectrometer (Waters, Milford, MA, USA) was used for LC-MS/MS analysis, and NMR JEOL ECZ-500 and Variant Unity INOVA-500 Spectrometer (Agilent Technologies, Santa Carla, CA, USA) were used to elucidate the structural compounds. The nuclear magnetic resonance (NMR) was recorded at 500 MHz for 1H and 150 MHz for 13C. Chemical shifts are given in δ (ppm) relative to TMS as internal standard, and deuterated chloroform was used as the solvent. The SARS-CoV-2 3CLpro #78042 assay kit was purchased from BPS Bioscience Inc., (San Diego, CA, USA).
G 25 uv254
Manufactured by Merck Group
Sourced in Germany, United States
The G-25 UV254 is a compact, high-performance laboratory instrument designed for the detection and quantification of nucleic acids and proteins. It utilizes ultraviolet (UV) light at a wavelength of 254 nm to generate an absorbance signal that is proportional to the concentration of the analyte. The G-25 UV254 provides a reliable and efficient method for various applications in molecular biology, biochemistry, and analytical chemistry.
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Lab products found in correlation
Silica gel 60, by Merck Group (2 mentions)
Variant unity inova 500 spectrometer, by Agilent Technologies (1 mentions)
Qp 2010 gas chromatograph mass spectrometer ultra, by Shimadzu (1 mentions)
Xevo g2 xs qtof mass spectrometer, by Waters Corporation (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
2 protocols using g 25 uv254
1
Phytochemical Analysis and SARS-CoV-2 3CLpro Inhibition
2
Isolation and Characterization of Terpenoids
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TLC aluminium sheets 20 × 20 cm Silica gel 60 F254 was used. Silica gel 60 (Merck) for vacuum liquid column chromatography (230-400 mesh) was used. Preparative precoated TLC glass plates SIL G-25 UV254, 0.25 mm silica gel and Sephadex LH-20 (Sigma, St. Louis, USA) were used for isolation and purification of the compounds. Spots on TLC were visualized by using spraying reagent of methanol-sulphuric acid and p-anisaldehyde-sulphuric acid for terpenoid detection. Nuclear magnetic resonance (NMR) were recorded for 1D and 2D on Avance III Bruker WM 600 MHz for 1 H and 150 MHz for 13 C. Chemical shifts are given δ (ppm) relative to TMS as internal standard, and deuterated chloroform was used as solvents. Electron impact mass spectra were determined at 70 eV using a Kratos GCMS-25 instrument.
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