Dual luciferase reporter gene uorescence detection kit

PTX was purchased from Aladdin. Dulbecco's Modi ed Eagle Medium (DMEM)/F12 was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Biological Industries. Trypsin, crystal violet, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, Cell Counting Kit-8, and P53 antibody were purchased from Beyotime Biotechnology. Trizol was purchased from Ambion. RevertAid First Strand complementary DNA (cDNA) Synthesis Kit was purchased from Thermo. SYBR Green Polymerase Chain Reaction (PCR) kit was purchased from Bimake. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Signalway Antibody. Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody was purchased from Santa Cruz Biotechnology. MDM2 antibody was purchased from A nity. Polyvinylidene di uoride (PVDF) membrane was purchased from Immobilon. 0.3% triton-X was purchased from Vetec. Lipofectamine®3000 transfection reagent was purchased from Invitrogen. MiRNA mimics and inhibitors were synthesized by GenePharma (Shanghai, China). Dualluciferase reporter vector plasmid was purchased by GenePharma (Shanghai, China). Dual-luciferase reporter gene uorescence detection kit was purchased by Promega.