Log In Sign up
The largest database of trusted experimental protocols

1 3 bis 1 2 dimyristoyl sn glycero 3 phospho sn glycerol

Manufactured by Avanti Polar Lipids
Visit Supplier
Sourced in United States

1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol is a synthetic lipid compound. It is a symmetric diglyceride molecule with two phosphatidylglycerol head groups. The compound can be used as a laboratory reagent.

Automatically generated - may contain errors

Lab products found in correlation

9 aminoacridine hemihydrate 9 aa, by Thermo Fisher Scientific (4 mentions) 1 2 dimyristoyl sn glycero 3 phosphate, by Avanti Polar Lipids (4 mentions) 1 2 dimyristoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (4 mentions) 1 2 diphytanoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (4 mentions) 1 2 dimyristoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (4 mentions) 1 3 bis 1 2 dioleoyl sn glycero 3 phospho sn glycerol, by Avanti Polar Lipids (3 mentions) 1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions) 1 2 dilauroyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) Ubiquitin, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphate, by Avanti Polar Lipids (1 mentions)

6 protocols using 1 3 bis 1 2 dimyristoyl sn glycero 3 phospho sn glycerol

1

Lipidomic Analysis by MALDI-TOF/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
All organic solvents used for lipid extraction and MS analyses were commercially distilled and of the highest available purity (Sigma–Aldrich). The matrix used for MALDI-TOF/MS analyses was the 9-Aminoacridine hemihydrate (9-AA) and was purchased from Acros Organics (Morris Plains, NJ, United States). The following commercial glycerophospholipids (used as standards): 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol), bis(monooleoylglycero)phosphate, 1-palmitoyl-2- linoleoyl-sn-glycero-3-phosphate, bis(monomyristoylglycero) phosphate, N-palmitoyl-D-erythro-sphingosylphosphorylcho- line, N-stearoyl-D-erythro-sphingosylphosphorylcholine, sn-(3- myristoyl-2-hydroxy)-glycerol-1-phospho-sn-3′-(1′,2′-dimyrist- oyl)-glycerol, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-gly- cerol), 1,2-dimyristoyl-sn-glycero-3-phosphate, 1,2-dimyristoyl- sn-glycero-3-phospho-L-serine, 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine, 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol, 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phos- pho]-sn-glycerol, were purchased from Avanti Polar Lipids, Inch (Alabaster, AL, United States). Plates for thin-layer chromatography (TLC) (Silica gel 60A, 10 × 20 cm, 0.2-mm-thick layer), were purchased from Merck (Darmstadt, Germany).
Lobasso S., Tanzarella P., Mannavola F., Tucci M., Silvestris F., Felici C., Ingrosso C., Corcelli A, & Lopalco P. (2021). A Lipidomic Approach to Identify Potential Biomarkers in Exosomes From Melanoma Cells With Different Metastatic Potential. Frontiers in Physiology, 12, 748895.
+ Open protocol
+ Expand
2

Phospholipid Reagents for Biophysical Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were used: 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (sodium salt) (14:0/14:0 PG); 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (12:0/12:0 PE) and 1’,3’-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (sodium salt) (14:0 Cl) purchased from Avanti Polar Lipids (Alabaster, AL, USA). The other chemicals were acquired from Sigma-Aldrich (Saint Louis, MO, USA), and POCh (Gliwice, Poland). All chemicals were high purity grade reagents.
Sadowska B., Laskowski D., Bernat P., Micota B., Więckowska-Szakiel M., Podsędek A, & Różalska B. (2019). Molecular Mechanisms of Leonurus Cardiaca L. Extract Activity in Prevention of Staphylococcal Endocarditis—Study on in Vitro and ex Vivo Models. Molecules, 24(18), 3318.
+ Open protocol
+ Expand
3

Glycerophospholipid Standards Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols
9-Aminoacridine hemihydrate (9-AA) was purchased from Acros Organics (Morris Plains, NJ). All organic solvents used in extraction and MS analyses were commercially distilled, of the highest available purity, and purchased from Sigma-Aldrich, J. T. Baker, or Carlo Erba. The following commercial glycerophospholipids (used as standards) 1,2-dimyristoyl-sn-glycero-3-phosphate, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol), 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine, 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine, 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol, 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-sn-glycerol, were purchased from Avanti Polar Lipids (Alabaster, AL).
Lopalco P., Stahl J., Annese C., Averhoff B, & Corcelli A. (2017). Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii. Scientific Reports, 7, 2972.
+ Open protocol
+ Expand
4

MALDI-TOF/MS Glycerophospholipid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The matrix used for MALDI-TOF/MS analyses was 9-aminoacridine hemihydrate (9-AA) and was purchased from Acros Organics (Morris Plains, NJ, United States). All organic solvents used in extraction and MS analyses were commercially distilled, of the highest available purity, and purchased from Sigma-Aldrich, J.T. Baker, or Carlo Erba. The following commercial glycerophospholipids (used as standards): 1,2-dimyristoyl-sn-glycero-3-phosphate, 1,2-dimyristoyl-sn-glycero- 3-phospho-(1′-rac-glycerol), 1,2-dimyristoyl-sn-glycero-3-phos- pho-L-serine, 1,2-diphytanoyl-sn-glycero-3-phosphoethanola- mine, 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glyce- rol, 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-sn-glycerol, were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, United States).
Lopalco P., Vitale R., Cho Y.S., Totaro P., Corcelli A, & Lobasso S. (2019). Alteration of Cholesterol Sulfate/Seminolipid Ratio in Semen Lipid Profile of Men With Oligoasthenozoospermia. Frontiers in Physiology, 10, 1344.
+ Open protocol
+ Expand
5

MALDI-TOF/MS Analysis of Glycerophospholipids

Check if the same lab product or an alternative is used in the 5 most similar protocols
The matrix used for MALDI-TOF/MS analyses was 9-Aminoacridine hemihydrate (9-AA) and was purchased from Acros Organics (Morris Plains, NJ, United States). All organic solvents used in extraction and MS analyses were commercially distilled, of the highest available purity, and purchased from Sigma-Aldrich, J. T. Baker, or Carlo Erba. The following commercial glycerophospholipids (used as standards): 1,2-dimyristoyl-sn-glycero-3-phosphate, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol), 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine, 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine, 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol, 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-sn-glycerol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, United States).
Lopalco P., Lobasso S., Lopes-dos-Santos R.M., Van Stappen G, & Corcelli A. (2019). Lipid Profile Changes During the Development of Artemia franciscana, From Cysts to the First Two Naupliar Stages. Frontiers in Physiology, 9, 1872.
+ Open protocol
+ Expand
6

Purification and Reconstitution of AmtB

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ubiquitin (bovine erythrocytes, >90% purity) was purchased from Sigma Aldrich (St. Louis, MO). A 5.0 μM stock solution in deionized water (18 MΩ) with 0.1% formic acid was prepared and used without further purification. Ammonia transport channel (AmtB) was expressed and purified as described previously [16 (link)]. AmtB was diluted in 200 mM ammonium acetate and 0.5% C8E4. Lipid samples of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate (POPA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), and 1′,3′-bis [1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (TMCDL) were purchased from Avanti Polar Lipids Inc. (Alabama, USA). Lipids stocks were prepared in 200 mM ammonium acetate and 0.5% C8E4 and used without further purification.
Poltash M.L., McCabe J.W., Patrick J.W., Laganowsky A, & Russell D.H. (2018). Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer. Journal of the American Society for Mass Spectrometry, 30(1), 192-198.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!