3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt

Magnesium(II) acetate tetrahydrate (99%), zinc(II) acetate dihydrate (99.5%), N-hydroxyl succinimide (97%), 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC∙HCl) extra pure (99%), 3-carboxybenzeneboronic acid (97%), potassium hydroxide pellets, disodium dihydrogen phosphate dihydrate, potassium dihydrogen phosphate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and sodium chloride were purchased from Sisco Research Laboratory (Mumbai, India). 3-aminopropyl triethoxysilane (APTES) with a purity of 99% was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Ethyl alcohol (EtOH) and dimethyl sulfoxide (DMSO) were purchased from Merck (Kenilworth, New Jersey, United States). Curcumin, DMEM media, RPMI-1640 media, amino acids and antibiotics were purchased from Hi-Media (Mumbai, India). Fetal bovine serum (FBS) was purchased from Thermo Scientific Hy-Clone (Logan, Utah, USA).

Mangiferin, Cisplatin, H₂-DCFDA, JC-1 and FITC conjugated Annexin V apoptosis detection kit was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, United States). Dulbecco’s Modified Eagle’s Medium (DMEM), MEM and other chemicals like antibiotics, trypsin etc. was purchased from HIMEDIA (Mumbai, India). Fetal bovine serum (FBS) was bought from HyClone (Thermo Scientific Hy-Clone, Logan, UT, United States).
The primers used for RT-PCR were purchased from IDT. The antibodies used were purchased from Abcam (Cambridge, United Kingdom), Cell Signaling Technology (Danvers, MA 01923, United States), BioBharati Lifescience (India). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) along with all other necessary chemicals and reagents were of analytical grade and purchased from the SISCO Research Laboratory, Mumbai, India.