Pi3k activity elisa kit k 1000s

Saos-2, MG-63 and SJSA-1 human OS cell lines and PK136 hybridoma cell line were purchased from the American Type Culture Collection (Manassas, VA, USA). ETAR shRNA lentiviral particles (sc-39960-V), control shRNA lentiviral particles-A (sc-108080), selective phosphatidylinositide 3-kinase (PI3K) inhibitor BKM120 (sc-364437A), anti-ETAR (sc-21193) antibody, anti-matrix metalloproteinase-2 (MMP-2) antibody (sc-53630), anti-Akt (5C10) (sc-81434) and anti-P-Akt (ser473) (sc-101629) antibodies, and a mouse anti-ganglioside GD2 mAb (14G2a) (sc-53831) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A mouse isotype-matched control PK136 mAb for 14G2a were purified from hybridoma culture supernatants using the Hi-Trap Protein G HP column (GE Healthcare Life Sciences, Shanghai, China) according to the manufacturer's protocol. The SensoLyte 520 MMP-2 Assay Kit (71151) was purchased from AnaSpec (Fremont, CA, USA). The QCM ECMatrix 24-well (8 µM) Fluorimetric Cell Invasion Assay kit (ECM554) was purchased from Chemicon (Millipore, Billerica, CA, USA). The ET-1 ELISA kit and the Methlythiazoletetrazolium (MTT) cell viability assay kit was purchased from R&D Systems (Minneapolis, MN, USA). The PI3K Activity ELISA kit (K-1000s) was purchased from Echelon Biosciences (Salt Lake City, UT, USA). Selective ETAR antagonist BQ123 was purchased from Sigma (St. Louis, MO, USA).