Fatostatin

Manufactured by ChemBridge

Fatostatin is a laboratory product that functions as an inhibitor of fatty acid synthase, a key enzyme involved in lipid biosynthesis. It can be used in research and experimental settings to study the role of fatty acid synthesis in various biological processes.

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Lab products found in correlation

Du145, by American Type Culture Collection (1 mentions) Filipin 3, by Merck Group (1 mentions) Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit 1, by BD (1 mentions) Cholesterol cholesterol ester detection kit, by Abcam (1 mentions) Free fatty acid quantification kit, by Abcam (1 mentions) Oil red o, by Merck Group (1 mentions) Ribonuclease a, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Matrigel basement membrane matrix, by BD (1 mentions)

2 protocols using fatostatin

Prostate Cancer Cell Lines and Genetic Manipulation

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Human metastatic prostate cancer PC-3 (p53-null) and DU145 (p53-mutant) cell lines were obtained from American Type Culture Collection (ATCC) and grown in RPMI-1640 medium supplemented with 10% fetal bovine serum (Atlanta biological, Flowery Branch, GA), penicillin (100 U/ml) and streptomycin (100 μg/ml) at 37°C in a humidified incubator with 5% CO₂. Human wtp53, various p53 mutants (V143A, R248W, R175H and R273H) and control expression constructs were obtained from Addgene (Cambridge, MA). Human p53 shRNA lentiviral particles were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Fatostatin and docetaxel were purchased from Chembridge Corporation (San Diego, CA) and Selleck Chemicals (Houston, TX), respectively.

Li X., Wu J.B., Chung L.W, & Huang W.C. (2015). Anti-cancer efficacy of SREBP inhibitor, alone or in combination with docetaxel, in prostate cancer harboring p53 mutations. Oncotarget, 6(38), 41018-41032.

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Fatostatin Modulates Lipid Metabolism

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Fatostatin (4-[4-(4-methylphenyl)-1,3-thiazol-2-yl]-2-propylpyridine hydrobromide) was purchased from Chembridge Corporation and its chemical structure was described in Supplementary Fig. S1. Ribonuclease A, propidium iodide (PI), Oil Red O and Filipin III were purchased from Sigma-Aldrich. Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit I and Matrigel Basement Membrane Matrix were purchased from BD Bioscience. The Free Fatty Acid Quantification Kit and Cholesterol/Cholesterol Ester Detection Kit were obtained from Abcam. pHMGCoASyn-Luc construct was obtained from Dr. Hitoshi Shimano of Tsukuba University. pFASN-700-Luc and pFASN-700-mutSRE-Luc constructs were obtained from Dr. Timothy F. Osborne of Sanford-Burnham Medical Research Institute. pLDLR-Luc and pLDLR-mutSRE constructs were obtained from Addgene (Cambridge, MA).

Li X., Chen Y.T., Hu P, & Huang W.C. (2014). Fatostatin Displays High Anti-Tumor Activity in Prostate Cancer by Blocking SREBP-Regulated Metabolic Pathways and Androgen Receptor Signaling. Molecular cancer therapeutics, 13(4), 855-866.

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