Phospho 4ebp1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Phospho-4EBP1 is a laboratory reagent used for the detection and quantification of phosphorylated 4E-binding protein 1 (4EBP1) in biological samples. 4EBP1 is a key regulator of protein synthesis, and its phosphorylation state is indicative of cellular signaling pathways. This product can be used in various research applications to study cellular processes related to protein translation and regulation.

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Lab products found in correlation

2 protocols using phospho 4ebp1

Letrozole and Estradiol Signaling Regulation

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Reagents used were as follows: letrozole (Sigma, L6545), 17β-estradiol (E2) (Sigma, 491187), celecoxib (Sigma, PZ0008), PGE2 (Sigma, P5640), tunicamycin (Sigma, T7765), salubrinal (Calbiochem, 324895), rapamycin (Sigma, R0395), arachidonic acid (Sigma, A9673), MTT (Sigma, M2128), Z-VAD-fmk (Sigma, V116), bafilomycin A1 (Sigma, B1793), 3-MA (Sigma, 08592), Hoechst 33342 (Sigma, B2261), acridine orange (Sigma, A6014), DMSO (Sigma, D2650). letrozole, E2, celecoxib, PGE2, tunicamycin, and salubrinal were dissolved in DMSO, while MTT, Hoechst 33342, and acridine orange were dissolved in phosphate-buffered saline (PBS).
Antibodies were obtained from the following sources: antibodies against Beclin 1, COX-2, EP-4, β-actin, Bcl-2, BAX, phospho-4EBP1 and phospho-Akt (S473) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); Phospho-p70-S6K(T389), eIF2α, phospho-eIF2α, Raptor, phospho-mTOR(S2448), caspase 3, and phospho-S6(S235/236) were from Cell Signaling Inc (Beverly, MA); LC3, ATG5, protein disulfide isomerase (PDI) were from Abcam (Cambridge, MA); horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody and horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Zhou S., Zhao L., Yi T., Wei Y, & Zhao X. (2016). Menopause-induced uterine epithelium atrophy results from arachidonic acid/prostaglandin E2 axis inhibition-mediated autophagic cell death. Scientific Reports, 6, 31408.

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Quantitative Western Blot Analysis

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Cells were lysed with M‐PER™ Mammalian Protein Extraction Reagent (Catalog #78503; Thermo Fisher) on ice for 30 min with vortexing and then sonicated with Q125 Sonicator from Qsonica (Newtown, CT, USA). Lysates were centrifuged at 18,407 g for 15 min at 4°C, and the protein concentration was measured using a BCA kit (Catalog #23225; Thermo Scientific). The cell lysates were adjusted to 5 mg mL⁻¹ to run on SDS‐PAGE. Following electrophoresis, proteins were transferred onto PVDF membranes and incubated with rabbit monoclonal antibodies against Aurora‐A, Phospho‐Aurora‐A, PLK1, Phospho‐PLK1 (Thr210), mTOR or Phospho‐mTOR (Ser2448) from Cell Signaling Technology (Danvers, MA, USA), or with monoclonal antibodies against 4E‐BP1 or Phospho‐4E‐BP1 from Santa Cruz Biotechnology (Dallas, TX, USA). ECL kit (Catalog #1705061) and the Gel Doc™ XR+ System from Bio‐Rad (Hercules, CA, USA) were used to develop and acquire Western blot images. Band intensity was analysed using the ImageJ software (NIH) and normalised by loading control β‐actin levels.

Li Y., Wang H., Zhang Z., Tang C., Zhou X., Mohan C, & Wu T. (2022). Identification of polo‐like kinase 1 as a therapeutic target in murine lupus. Clinical & Translational Immunology, 11(1), e1362.

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