ABK5-1 was purchased from ChemBridge Corporation (San Diego, CA USA). Lipopolysaccharide (LPS) was obtained from Millipore Sigma (St. Louis, MO USA). Phospho-p44/42 MAPK (pERK) and p44/42 (ERK) rabbit monoclonal antibodies were obtained from Cell Signaling Technology (Danvers, MA USA). IRDye 680RD Goat anti-Rabbit IgG (H + L) was purchased from LI-COR Biotechnology (Lincoln, NE USA).
Phospho p44 42 mapk perk
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-p44/42 MAPK (pERK) is a lab equipment product that detects the phosphorylated forms of p44/42 MAPK (Erk1/Erk2). It is used to measure the activation of the p44/42 MAPK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using phospho p44 42 mapk perk
1
Immunoblotting with ABK5-1 and LPS
2
Immunoblotting with ABK5-1 and LPS
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ABK5-1 was purchased from ChemBridge Corporation (San Diego, CA USA). Lipopolysaccharide (LPS) was obtained from Millipore Sigma (St. Louis, MO USA). Phospho-p44/42 MAPK (pERK) and p44/42 (ERK) rabbit monoclonal antibodies were obtained from Cell Signaling Technology (Danvers, MA USA). IRDye 680RD Goat anti-Rabbit IgG (H + L) was purchased from LI-COR Biotechnology (Lincoln, NE USA).
3
Larval lysate Western blot analysis
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Larval lysates were prepared as previously described [29 (link)] and examined using 10% SDS–PAGE. The gel transferred to the nitrocellulose membrane was analysed with antibodies against p44/42 MAP kinase (ERK 1/2; ERK; 1 : 1000; Cell Signaling, EuroClone, Milan, Italy) and Phospho-p44/42 MAPK (P-ERK; 1 : 500; Cell Signaling). After washing in PBS with 0.1% Tween, labelled proteins were detected by ECL PLUS (GE Healthcare, EuroClone, Milan, Italy).
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