Total RNA was isolated from SYK-6 cells grown in Wx medium containing 10 mM VA and SYK-6 cells harboring pKTDBP, which carried the desB promoter region, grown in Wx medium containing 10 mM SA. cDNAs were synthesized from total RNA (5 and 20 µg) with Beckman dye D4-labeled PEdesB (2 pmol) and D2-labeled PEligM (2 pmol), respectively, using a PrimeScript 1st Strand cDNA Synthesis Kit. The extended products were purified by phenol-chloroform extraction and ethanol precipitation then dissolved in CEQ Sample Loading Solution (Beckman Coulter, Fullerton, CA, USA) combined with DNA Size Standard Kit 400 (Beckman Coulter). Samples were analyzed utilizing a CEQ 2000XL genetic analysis system (Beckman Coulter).
Ceq 2000xl genetic analysis system
Manufactured by Beckman Coulter
Sourced in United States
The CEQ 2000XL is a genetic analysis system designed for DNA sequencing and fragment analysis. It utilizes capillary electrophoresis technology to separate and detect fluorescently labeled DNA samples. The system provides automated data collection and analysis for a range of molecular biology applications.
Automatically generated - may contain errors
2 protocols using ceq 2000xl genetic analysis system
1
Quantitative mRNA Analysis in SYK-6 Cells
2
Capillary Electrophoresis for Fragment Analysis
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Samples were resolved by capillary electrophoresis separation, using a CEQ 2000 XL genetic analysis system (Beckman Coulter, Inc. CA, USA). To 0.5 µL of PCR product were added 0.25 µL of a 600 bp standard and 40 µL of sample loading solution (Beckman Coulter). Specifically designed labeled oligonucleotides were excited to fluoresce using a diode laser. The length of the amplified fragment was estimated with reference to the internal ladder and the number of repeats was calculated by analogy with 10 sequenced samples, using the CEQ 8000 Beckman Coulter software provided.
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