Sotrastaurin aeb071

The R97-116 peptide derived from the rat (AChRα) subunit was used as an immunogen. The peptide (DGDFAIVKFTKVLLDYTGHI) was obtained from Bankpeptide Technology Co. (Hefei, China) (MW: 2252.57 Da, purity >95%). Mycobacterium tuberculosis H37RA powder was purchased from DIFCO (Franklin Lakes, NJ, USA). Freund’s adjuvants were obtained from Sigma (St. Louis, MO, USA.). Rat AChR-Ab ELISA test kits (QS41981) were purchased from Qisong Biotechnology Co. (Beijing, China). Sotrastaurin (AEB-071) was produced by GLPBIO (Montclair, CA, USA). Antibodies (anti-rat CD4-FITC, CD25-PE, and Foxp3-APC), fixation buffer, true-nuclear transcription factor buffer sets, intracellular staining permeabilization buffer, and leukocyte cell activation cocktail were purchased from Bio Legend, Inc. Erythrocyte Lysis Buffer and rat interleukin (IL)-17A-APC were purchased from eBioscience, Inc. (San Diego, CA, USA) Azathioprine (AZP) and 0.9% saline (NS) were provided by the Pharmacy Department of the 8th Medical Center of Chinese PLA General Hospital. RPMI-1640 was prepared in-house. The optical density of the test sample and the standard was determined with a Bio-Kinetics microplate reader at a wavelength of 450 nm. Flow cytometry was performed on a BD LSRII flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).