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Planneofluar z 1x 0.25 fwd 56 mm objective

Manufactured by Zeiss

The PlanNeoFluar Z 1x/0.25 FWD 56 mm objective is a high-quality microscope objective designed for a wide range of applications. It offers a 1x magnification and a numerical aperture of 0.25, providing a working distance of 56 mm. This objective is part of the PlanNeoFluar series, known for its excellent optical performance and durability.

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2 protocols using planneofluar z 1x 0.25 fwd 56 mm objective

1

Ectopic Angiogenesis Analysis in Zebrafish

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mttpstl/stl, mttpc655/c655 and WT zebrafish in the Tg(fli1:eGFP)y1 background were imaged at 3 dpf with a Zeiss Axiozoom V16 microscope equipped with a Zeiss PlanNeoFluar Z 1x/0.25 FWD 56 mm objective, AxioCam MRm camera, and Zen 2.5 software. The length of ectopic angiogenic segments that extend from the subintestinal vessels were analyzed in Fiji [104 (link)](ImageJ V2.0.0, National Institutes of Health (NIH), USA) as described by [49 (link)].
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2

ABA-Induced Root Growth Inhibition Assay

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Seedlings were grown for six days on plates containing growth medium with the same composition as the liquid medium but containing in addition 0.8% Agarose (Sigma). Seedlings were transferred from regular plates to plates either 10 µM ABA or no ABA (GM). Positions of root tips were marked after transfer and then roots were allowed to grow for 24 hours. After 24 hours images were taken using a Zeis Axio Zoom.V16 equipped with a PlanNeoFluar Z 1x/0.25 FWD 56mm objective lens, a 25x/10foc eyepiece and a Zeiss Axiocam506 colour.
Root lengths were determined using the freehand drawing tool in Fiji Version 2.1.0. Three independent experiments were performed and for each treatment / genotype combination >15 seedling roots were measured per experiment. For calculation of relative root length, the following formula was applied:
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