Tissues of male HF/HCD-fed control and Liv-Lipa−/− mice were fixed in 4% neutral-buffered formaldehyde for 24 h and embedded in paraffin. Sections (5 μm) were de-paraffinized and subjected to chromotrope aniline blue (CAB) and hematoxylin and eosin (HE) staining, respectively, using standard histopathological techniques [29 (link)]. For macrophage staining, sections were incubated with anti-mouse F4/80 antibody (1:50; Serotec MCA 497GA; Biorad, Hercules, CA). Antibody binding was visualized using aminoethyl carbazole substrate chromogen (cat#3464; Dako, Glostrup, Denmark). For neutral lipid staining, 7 μm cryosections were stained with Oil Red O (ORO) and nuclei were counter-stained with Mayer's hematoxylin.
Aminoethyl carbazole substrate chromogen
Manufactured by Agilent Technologies
Sourced in Denmark
Aminoethyl carbazole substrate chromogen is a chemical compound used as a substrate in various laboratory applications, particularly in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA). It functions as a chromogen, producing a colored reaction product when catalyzed by an enzyme, enabling the visualization and detection of target analytes or molecules of interest.
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Lab products found in correlation
3 protocols using aminoethyl carbazole substrate chromogen
1
Histological Analysis of Liver Lipid Metabolism
2
Histological Analyses of Liver Tissues
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Livers of standard chow and VitA/HFD-fed hep-LAL-ko mice and WT littermates were fixed in 4% neutral-buffered formaldehyde for 48 h and embedded in paraffin. Sections (5 μm) were de-paraffinized and subjected to hematoxylin and eosin (H&E) or CAB trichrome staining, respectively, using standard histological techniques (45 (link)). Neutral lipids were stained in cryosections of formaldehyde-fixed, nonembedded tissues using ORO, and nuclei were counter-stained with Mayer's hematoxylin. For macrophage staining, formaldehyde-fixed, paraffin-embedded sections were incubated with anti-mouse F4/80 antibody (1:50; Serotec MCA 497GA; Bio-Rad). Antibody binding was visualized using aminoethyl carbazole substrate chromogen (catalog no. 3464; Dako, Glostrup, Denmark). Stained surface areas were quantified with ImageJ software (46 ) and normalized to total inspected surface area.
3
Histological Analysis of Liver Lipid Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues of male HF/HCD-fed control and Liv-Lipa−/− mice were fixed in 4% neutral-buffered formaldehyde for 24 h and embedded in paraffin. Sections (5 μm) were de-paraffinized and subjected to chromotrope aniline blue (CAB) and hematoxylin and eosin (HE) staining, respectively, using standard histopathological techniques [29 (link)]. For macrophage staining, sections were incubated with anti-mouse F4/80 antibody (1:50; Serotec MCA 497GA; Biorad, Hercules, CA). Antibody binding was visualized using aminoethyl carbazole substrate chromogen (cat#3464; Dako, Glostrup, Denmark). For neutral lipid staining, 7 μm cryosections were stained with Oil Red O (ORO) and nuclei were counter-stained with Mayer's hematoxylin.
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