Linco human insulin specific radioimmunoassay kit

Serum collected at the baseline examination after a minimum 8-h fast was used to measure fasting insulin and glucose. Glucose was measured using the Vitros 950 analyzer (Johnson & Johnson Ortho-Clinical Diagnostics), and insulin was measured using the Linco Human Insulin Specific Radioimmunoassay kit (Linco Research, Inc.).

Cross-sectional IR was assessed by fasting plasma glucose levels, insulin levels, and HOMA-IR. Fasting blood glucose in serum was measured by the glucose oxidase method using the Vitros 950 analyzer (Johnson & Johnson Clinical Diagnostics, Rochester, New York). Fasting serum insulin was measured by the Linco Human Insulin Specific Radioimmunoassay kit (Linco Research, Inc., St. Charles, Missouri) [34 (link)]. HOMA-IR was calculated using the equation: glucose (mg/dL) x insulin (mU/L) / 405. Prediabetes was defined according to the American Diabetes Association as fasting glucose ≥100 & <126 mg/dL. Incident type 2 diabetes was accessed prospectively over a median 10 years of follow-up and was defined as a fasting glucose ≥7.0 mmol/l (126 mg/dl) and/or use of insulin or oral hypoglycemic medications.