Following fixation in 4% PFA, samples were embedded in 1% agarose by boiling and slowly cooling down to room temperature. Samples processed with BABB were sequentially incubated in 25, 50, 80, and 100% ethanol (Merck Millipore, Burlington, MA, United States) for 8 h each at room temperature. Samples processed with 1-propanol BABB (1P-BABB) were sequentially incubated in 25, 50, 80, and 100% 1-propanol (Merck Millipore, Burlington, MA, United States) for 8 h each at room temperature. Samples processed with tert-butanol BABB (tB-BABB) were sequentially incubated in 25, 50, 80, and 100% tert-butanol (Daejung Chemical & Metals Co. Ltd., Gyeonggi-do, Korea) for 8 h each at room temperature. After alcohol dehydration, the samples were incubated in fresh solutions of either 100% ethanol, 1-propanol, or tert-butanol, as appropriate, for 12 h at room temperature. Subsequently, samples were incubated in dichloromethane (DCM; Sigma-Aldrich Inc., St. Louis, MO, United States) for 1 h at room temperature. Samples were then incubated in BABB solution [1 volume of benzyl alcohol (BA; Sigma-Aldrich Inc., St. Louis, MO, United States) to 2 volumes of benzyl benzoate (BB; Duksan Pure Chemicals, Gyeonggi-do, Korea)] for 1–2 days until they achieved optical transparency.
Benzyl alcohol ba
Manufactured by Merck Group
Sourced in Macao, United States
Benzyl alcohol (BA) is a clear, colorless liquid used as a solvent and preservative in various laboratory applications. It is a versatile compound that serves as a chemical intermediate in the synthesis of various organic compounds. BA has a distinctive aromatic odor and is commonly used in the formulation of personal care products, pharmaceuticals, and other industrial applications.
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Lab products found in correlation
2 protocols using benzyl alcohol ba
1
Clearing and Transparency Enhancement of Biological Samples
2
Heat Treatment in Resistometer with Benzyl Alcohol
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Heat treatments were carried out in a specially designed resistometer (Condón et al., 1993) . Briefly, this instrument consists in a 350-mL vessel provided with an electrical heater for thermostation, an agitation device to ensure inoculum distribution and temperature homogeneity, and ports for the injection of microbial suspension and for the extraction of samples. Once treatment temperature had attained stability (±0.1 °C), 0.2 mL of an appropriately diluted cell suspension was injected into the main chamber containing the treatment media. After inoculation, samples were collected at different treatment times and immediately pour-plated (Condón et al., 1996) . The treatment medium was McIlvaine citrate-phosphate buffer of pH 7.0 (Dawson et al., 1974) .
In some experiments, benzyl alcohol (BA) (Sigma, St. Louis, USA) was added as a membrane fluidizer to the cell suspensions and to the treatment medium at concentrations ranging from 5 mM to 20 mM (Cebrián et al., 2016; Shigapova et al., 2005) .
In some experiments, benzyl alcohol (BA) (Sigma, St. Louis, USA) was added as a membrane fluidizer to the cell suspensions and to the treatment medium at concentrations ranging from 5 mM to 20 mM (Cebrián et al., 2016; Shigapova et al., 2005) .
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