Rats were randomly classified into four groups (n = 8), including control group, LPS group, LPS + CAR25 group, and LPS + CAR50 group. Carvacrol-treated groups received Carvacrol intraperitoneally at doses of 25 and 50 mg/kg every day.[25 (link)] Carvacrol (Sigma-Aldrich Co., USA) was emulsified with 1% Tween 80 and dissolved in normal saline. The control and LPS groups received 1% Tween 80 dissolved in saline at the same volume as the treated groups. Injection of Carvacrol or 1% Tween was started 2 weeks before LPS injection and continued during LPS injections.[26 (link)]
LPS from Escherichia coli (Sigma-Aldrich Co., USA) was freshly prepared in saline and was injected intraperitoneally at a dose of 1 mg/kg, 2 h before behavioral testing[26 (link)] in the Morris water maze (MWM), which was performed on days 15–19. Treatments with Carvacrol or 1% Tween 80 were performed 30 min prior to behavioral task. After behavioral testing, rats were euthanized and their brains were immediately removed. The hippocampus and cerebral cortex were dissected, weighed, and homogenized with NaCl solution 10 times (w/v) for biochemical assessments. All chemicals for biochemical measurements (thiobarbituric acid reactive substances [TBARS] and total thiol concentration) were purchased from Merck Co. (Germany).
LPS from Escherichia coli (Sigma-Aldrich Co., USA) was freshly prepared in saline and was injected intraperitoneally at a dose of 1 mg/kg, 2 h before behavioral testing[26 (link)] in the Morris water maze (MWM), which was performed on days 15–19. Treatments with Carvacrol or 1% Tween 80 were performed 30 min prior to behavioral task. After behavioral testing, rats were euthanized and their brains were immediately removed. The hippocampus and cerebral cortex were dissected, weighed, and homogenized with NaCl solution 10 times (w/v) for biochemical assessments. All chemicals for biochemical measurements (thiobarbituric acid reactive substances [TBARS] and total thiol concentration) were purchased from Merck Co. (Germany).