GBM8401 cells were used to assess cell migration by a wound healing assay culture-insert (ibidi) for six-well plates. The insert was coated with cells seeded at 1 × 105 per insert and cultured at 37 °C for 12 h. si-GSK3B, si-galectin-3, and non-siRNA were added after 24 h. After 1 day, the plates were washed twice with PBS and photographed.
Wound healing assay culture insert
Manufactured by Ibidi
The Wound healing assay culture-insert is a lab equipment designed to create a reproducible in vitro wound for the purpose of studying cell migration and wound healing processes. It consists of two silicone inserts placed in a culture dish, allowing the creation of a defined cell-free gap. This gap serves as the 'wound' that can be monitored over time as cells migrate to close the space.
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Lab products found in correlation
2 protocols using wound healing assay culture insert
1
GBM8401 Cell Migration by Wound Healing
2
Wound Closure Kinetics Assay
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Previously transfected cells were passed into wound-healing assay culture insert (ibidi) 24 h after transfection. After 24 h of culture in the device, the insert was removed to make a wound of ∼450 µm. Images were taken at three different locations along the wound at every hour for 9 h. The distance between both sides of the wound was measured to calculate the speed of closure over the 9 h. For staining experiments, cells were fixed 6 h after removing the insert.
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