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Jem 1220 tem

Manufactured by JEOL
Sourced in Japan, United States

The JEM-1220 TEM is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of materials at the nanoscale level. The instrument utilizes an electron beam to illuminate and interact with the sample, allowing for detailed observation and characterization of the sample's structure and composition.

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28 protocols using jem 1220 tem

1

Characterization of Graphene Oxide Aqueous Solution

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A GO water solution (4mg/mL) was obtained from Advanced Graphene Products (Zielona Gora, Poland). In the experiment, dilutions were prepared in deionized water and sonicated for 15 minutes before use.
The MIR spectrum of the GO water solution was registered with KRS round plates in transmission mode. Five separate spectra of the working sample were registered, and an averaged spectrum was calculated. The Perkin Elmer System 2000 spectrometer was used for spectra registration, and analysis was performed in the Pegrams software.
The morphology of the GO flakes was examined using a TEM - JEM-1220 (JEOL, Japan) at 80 kV and a TEM CCD Morada 11 megapixels camera (Olympus Soft Imaging Solutions, Germany). The zeta potential of the GO solution was measured using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, UK).
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2

Characterization of Graphene Oxide

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Single layer of graphene oxide, as water dispersion 2 wt.%, was purchased from US Research Nanomaterials, Inc. (Houston, TX, USA). GO flakes had thickness of 0.43–1.23 nm and diameter of 1.5–5.5 μm (manufacturer’s data).
The morphology of GO was characterized using a TEM-JEM-1220 (JEOL, Tokyo, Japan) at 80 kV and a TEM CCD Morada 11-megapixel camera (Olympus Soft Imaging Solutions, Munster, Germany). The zeta potential of GO solution was measured by light scattering using a ZetaSizer Nano ZS model ZEN3500 (Malvern Instruments, Malvern, UK). All measurements were performed in four repetitions. For the experiment, GO was diluted in ultrapure Milli-Q water at the final concentration of 100 ppm and sonicated in an ultrasonic bath (Bandelin Electronic, Berlin, Germany).
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3

Morphological Characterization of GO and GO-miRNA-21

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The shape and size of the GO, antisense miRNA-21, and GO-miRNA-21 were inspected using a transmission electron microscope (TEM). The morphology of GO and GO-miRNA-21 was inspected using a transmission electron microscopy (TEM.JEOL JEM-1220, JEOL Ltd., Tokyo, Japan) at 80 KeV equipped with an 11-megapixel camera (Morada TEM, Olympus Corporation, Tokyo, Japan). Triplicate samples of GO and GO-antisense miRNA-21 were prepared for TEM by placing droplets of the hydrocolloid onto Formvar-coated copper grids (Agar Scientific Ltd., Stansted, UK) and air drying before TEM imaging.
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4

Particle Characterization via TEM

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To study the particles’ morphology, primary size, and agglomeration state, a drop (5 µL) of the following particle suspensions was added in ultrapure water: DEP at 100 µg/mL, CuO and ZnO at 20 µg/mL, and mixtures of DEP (100 µg/mL) and CuO or ZnO (20 µg/mL) were pipetted onto Formvar®-coated 200 mesh copper grids. The excess of water was gently blotted by filter paper. Once dried, grids were observed under the TEM Jeol JEM-1220 (JEOL, Tokyo, Japan) operating at 80 kV, equipped with a charged-coupled device (CCD) camera.
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5

Exosome Characterization by TEM and Western Blot

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Exosome pellets purified from the cells were firstly examined by TEM to ascertain the presence of exosomal vesicles, whose diameter has to be 100 nm or less [27 (link), 28 (link)]. Pellets were suspended in residual fluid from PBS wash, followed by addition of 100 μl freshly made fixative (2.5% glutaraldehyde in PBS) to preserve vesicle structure and morphology. Preparations were mounted on formvar nickel 300-mesh grids by layering grids over 10 ml drops of exosome preparations for 10 min at 24°C. Grid-mounted preparations were stained with uranyl acetate and lead citrate, and subsequently examined with a JEOL JEM 1220 TEM at 120 kV. Also, the presence of exosomes was confirmed by Alix detection using Western Blotting as described below.
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6

Transmission Electron Microscopy of Vesicular Membrane Structure

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The VM samples for transmission micrographs were fixed for 2 h in 2.5% glutaric aldehyde (C5H8O2) and then for 2 h in phosphate-buffered saline (PBS) (pH = 7.2; 4 °C). The final fixation was carried out with 1% OsO4 at 4 °C for 1 h. After dehydration in increasing the gradient of ethanol and acetate saturation, the samples were immersed in Epona (812). After polymerization, the VM preparations were cut with a diamond knife on an ultramicrotome (LKB, Sweden) and applied to copper nets, which were then contrasted in uranyl acetate and lead citrate. The VM structure image (TEM) was observed using an electron microscope (JEM 1220 TEM, JEOL, Japan). Selected TEM images were then transferred to a computer and measurements were obtained in Nis Elements, version 5.10. The number of layers forming OL and IL was counted. The thickness of the whole VM, OL, and IL layers was measured and any species-specific structures were marked.
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7

Ultrastructure Examination of Breast Muscle

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For ultrastructure examination, small specimens were immediately collected from breast muscle after dissection, tissues were cut into small pieces (about 1 mm3) and fixed for 3 h in 3% glutaraldehyde solution (Merck, Darmstadt, Germany) in 0.1 M sodium phosphate buffer (pH 7). Following, specimens were washed in two consecutive changes in buffer and then moved to a 1% osmium tetroxide solution (Electron Microscope Science, Sigma-Aldrich) for 1 h in 0.1 M phosphate buffer (pH 6.9). After that, tissue samples were washed for 5 min in 0.1 M sodium phosphate buffer, dehydrated in ascending grade of ethyl alcohol, and impregnated with Epon embedding resin. Then, samples were embedded and blocked at 60 °C for 48 h. Semi-thin sections were cut from the prepared blocks and stained with 1% basic Toluidine blue for the light microscopy. Following, ultrathin sections (50–80 nm) were prepared from the selected regions and moved onto copper grids (200 meshes). Finally, uranyl acetate dihydrate (2%) and lead citrate were used for sections contrasts. Tissues were examined and captured blindly by experienced pathologist (AFK) using JEM-1220 TEM (JEOL, Tokyo, Japan), with a Morada 11 megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany).
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8

Ultrastructural Analysis of Breast Muscle

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Immediately after slaughter, small specimens were obtained from breast muscle. Specimens were cut into small pieces (about 1 mm3) and immediately fixed in 3% glutaraldehyde solution (Merck, Darmstadt, Germany) for at least 3 h in 0.1 M PBS (pH 7). Fixed samples were washed in two changes of buffer and transferred to a 1% osmium tetroxide solution (Electron Microscope Science, Sigma-Aldrich) for 60 min in 0.1 M PBS (pH 6.9). Then, samples were rewashed in 0.1 M PBS for 5 min, dehydrated in increasing concentrations of ethanol, and impregnated with Epon embedding resin. Samples were embedded at 60 °C for 48 h and blocked. Semi-thin sections were prepared and stained with 1% basic Toluidine blue for light microscopy examination. After that, ultrathin Sects. (50–80 nm) were processed from the selected areas and placed onto copper grids (200 mesh). Finally, section contrasting was performed using uranyl acetate dihydrate (2%) and lead citrate. Tissues were examined and captured by JEM-1220 TEM (JEOL, Tokyo, Japan), with a Morada 11-megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany).
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9

Nanoparticle Characterization Protocol

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The particle size and distribution of tES(+)F116H and tES–F116H/POI were determined by dynamic light scattering (DLS) (Nanobrook Omni, Brookhaven), using disposable cuvettes at 24 °C and the average hydrodynamic diameter was determined by taking an arithmetic average of 10 runs. The morphologies of the nanoparticles were analyzed using transmission electron microscopy (TEM, JEOL JEM-1220 TEM) and their zeta potentials were measured using Zeta-Plus analyzer (Nanobrook Omni, Brookhaven).
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10

Ultrastructural Analysis of Avian Eggshells

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Six eggs from each of the four species were analyzed yielding a total of 24 samples. VM samples were fixed in 2.5% glutaraldehyde for 2 h at 4°C. The fixed samples were washed with phosphate buffer (pH 7.2) for about 2 h at 4°C. Then, the samples were fixed in 2.5% glutaraldehyde for 2 h at 4°C. The fixed samples were washed with phosphate buffer (pH 7.2) for about 2 h at 4°C. Then, the samples were fixed in 1% OsO4 at 4°C for 1 h and dehydrated in an increasing gradient of ethanol and saturated with acetone. Then, the samples were immersed in Epon 812. Following polymerization of the Epon, the samples were cut with a diamond knife on an ultramicrotome (LKB, Sweden) and transferred to copper nets, which were then contrasted in uranyl acetate and lead citrate. The prepared material was examined under TEM (JEM 1220 TEM, JEOL, Japan). From the TEM image, the thickness of the samples was measured and the number of layers of individual bird species was counted using the Nikon optical microscope (type 104c, Japan) equipped with Nis Elements, version 5.10.
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