Qscript cdna mastermix

Manufactured by Quanta Biosciences

QScript cDNA mastermix is a ready-to-use solution for reverse transcription and real-time PCR amplification. It contains all the necessary reagents for cDNA synthesis and quantitative PCR in a single tube, optimized for reliable and sensitive gene expression analysis.

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Lab products found in correlation

Mini cycler, by Bio-Rad (1 mentions) Superscript 4 reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Power sybr green, by Thermo Fisher Scientific (1 mentions) Miniopticon real time pcr system, by Bio-Rad (1 mentions) Viaa 7 real time pcr system, by Thermo Fisher Scientific (1 mentions) Rneasy 96 kit, by Qiagen (1 mentions) Taqman universal master mix, by Thermo Fisher Scientific (1 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

3 protocols using qscript cdna mastermix

Gene Expression Analysis by qPCR

Cited 1 time

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RNA was isolated from cells using Trizol (Invitrogen) and reverse transcribed using the SuperScript IV Reverse Transcriptase kit (Invitrogen) or QScript cDNA mastermix (Quanta BioSciences) according to the manufacture’s guidelines. cDNA reactions were carried out using a MiniCycler (MJ Research).
qPCR was carried out using Power Sybr Green (Invitrogen) on a MiniOpticon Real-Time PCR System (Bio-Rad), and the relative expression was calculated as previously described [12 (link)]. β-actin was used to normalize all samples. Sample expression was then determined relative to the no stimulation control. The primers used in this study have been reported previously [6 , 42 (link)].

Poe C., Youngblood C., Hodge K, & Kemp K. (2019). Treatment of established TH2 cells with 4μ8c, an inhibitor of IRE1α, blocks IL-5 but not IL-4 secretion. BMC Immunology, 20, 3.

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Quantitative qPCR Analysis of T Cell RNA

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Total RNA was isolated from cultured T cells with the RNeasy 96 kit (Qiagen; Germantown, MD) and reverse transcribed into cDNA with qScript cDNA mastermix (Quanta Biosciences; Gaithersburg, MD). qPCR was performed with Taqman primer-probe sets and Taqman universal mastermix (Applied Biosystems). Data were collected on the Viaa7 Real-Time PCR system (Applied Biosystems^™) and analyzed by the comparative Ct method (ΔΔCt) with normalization to the mean Ct of endogenous control genes HPRT1, TBP, and IPO8.

Mamontov P., Eberwine R.A., Perrigoue J., Das A., Friedman J.R, & Mora J.R. (2019). A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiation. PLoS ONE, 14(4), e0215963.

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Quantifying Human Fibroblast IGF2 and PLAG1

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Total RNA extraction from human fibroblast cells was carried out using TRIzol reagent (Invitrogen, Thermo Fisher Scientific). cDNA synthesis was performed using 1 μg of RNA and qScript cDNA master mix (Quanta Biosciences). Quantitative real-time polymerase chain reaction (qPCR) was performed utilizing aforementioned human IGF2 and PLAG1 primers with amplification by PowerUp SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific). Gene expression analysis was performed by the comparative CT (ΔΔCT) method using QuantStudio Design & Analysis software. Samples were normalized to the expression of GAPDH.

Maharaj A.V., Cottrell E., Thanasupawat T., Joustra S.D., Triggs-Raine B., Fujimoto M., Kant S.G., van der Kaay D., Clement-de Boers A., Brooks A.S., Aguirre G.A., Martín del Estal I., Castilla de Cortázar Larrea M.I., Massoud A., van Duyvenvoorde H.A., De Bruin C., Hwa V., Klonisch T., Hombach-Klonisch S, & Storr H.L. (2024). Characterization of HMGA2 variants expands the spectrum of Silver-Russell syndrome. JCI Insight, 9(6), e169425.

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