Nlrp3 and asc

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NLRP3 and ASC are proteins involved in the formation of the NLRP3 inflammasome, a multiprotein complex that plays a key role in the activation of the immune response. NLRP3 is a sensor that detects various danger signals, while ASC is an adapter protein that links NLRP3 to the effector caspase-1. These proteins are important targets for the study of inflammatory processes and their potential involvement in various diseases.

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Lab products found in correlation

Il 1β, by Abcam (4 mentions) Caspase 1, by Cell Signaling Technology (3 mentions) Actin, by Santa Cruz Biotechnology (2 mentions) Poly 1 c, by InvivoGen (2 mentions) Phospho p65, by Cell Signaling Technology (1 mentions) Phospho iκbα, by Cell Signaling Technology (1 mentions) Actin, by Merck Group (1 mentions) Cd4 and cd8, by BioLegend (1 mentions) Nkg2d, by Abcam (1 mentions) Recombinant murine ifn γ, by Thermo Fisher Scientific (1 mentions) Cxcl10, by R&D Systems (1 mentions) Z yvad fmk, by Abcam (1 mentions) Z levd fmk, by Abcam (1 mentions) Hmgb1, by Abcam (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Cox 4, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Sqstm1, by Abcam (1 mentions) Pgc 1α, by Abcam (1 mentions) Sod2 assay kit, by Beyotime (1 mentions)

4 protocols using nlrp3 and asc

Western Blot Analysis of Immune Proteins

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Cellular extracts were prepared using a lysis buffer (Intron, Daejeon, Korea). The cellular proteins were separated by gel electrophoresis and then transferred to nitrocellulose membranes. The membranes were incubated with primary antibody, and then sequentially incubated with peroxidase-conjugated secondary antibodies. The Western bands were obtained by enhanced chemiluminescence (Intron). The following primary antibodies were used: phospho-p65, phospho-IκBα, caspase-1 (Cell Signaling Technology, Beverly, MA, USA); IL-1β (Abcam, Cambridge, MA, USA); NLRP3 and ASC (AdipoGen, San Diego, CA, USA); actin (Sigma-Aldrich).

Zhang Q.L., Jiang R.H., Li X.M., Ko J.W., Kim C.D., Zhu M.J, & Lee J.H. (2019). Inhibition of Poly(I:C)-Induced Inflammation by Salvianolic Acid A in Skin Keratinocytes. Annals of Dermatology, 31(3), 279-285.

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Immunohistochemical Analysis of Immune Markers

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Poly(I:C) was obtained from InvivoGen (San Diego, CA). Recombinant murine IFNγ was obtained from Peprotech (315-05, Rocky Hill, NJ). For immunohistochemical analysis and western blotting, we used the following specific antibodies: CD4 and CD8 (Biolegend, San Diego, CA), CXCL10 (R&D systems, Minneapolis, MN), IL-1β (Abcam, Cambridge, MA), caspase-1 (Cell Signaling Technology, Danvers, MA), NLRP3 and ASC (Adipogen, San Diego, CA), NKG2D (Abcam, Cambridge, MA) and actin (Santa Cruz Biotechnologies, Santa Cruz, CA).

Shin J.M., Choi D.K., Sohn K.C., Koh J.W., Lee Y.H., Seo Y.J., Kim C.D., Lee J.H, & Lee Y. (2018). Induction of alopecia areata in C3H/HeJ mice using polyinosinic-polycytidylic acid (poly[I:C]) and interferon-gamma. Scientific Reports, 8, 12518.

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Inflammasome Activation Mechanism Study

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Poly(I:C) was obtained from InvivoGen (San Diego, CA). caspase-1 inhibitor (Z-YVAD-FMK) and caspase-4 inhibitor (Z-LEVD-FMK) were purchased from BioVision (Milpitas, CA). For immunohistochemical analysis and western blotting, we used the following specific antibodies: IL-1β and HMGB1 (Abcam, Cambridge, MA), caspase-1 (Cell Signaling Technology, Danvers, MA), NLRP3 and ASC (Adipogen, San Diego, CA), and actin, α-tubulin and laminB (Santa Cruz Biotechnologies, Santa Cruz, CA).

Shin J.M., Choi D.K., Sohn K.C., Kim S.Y., Min Ha J., Ho Lee Y., Im M., Seo Y.J., Deok Kim C., Lee J.H, & Lee Y. (2017). Double-stranded RNA induces inflammation via the NF-κB pathway and inflammasome activation in the outer root sheath cells of hair follicles. Scientific Reports, 7, 44127.

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Antibody-Guided Protein Analysis

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Antibodies recognizing the following proteins were used in this study: caspase-3, Bax, LC3, ATG7, COXIV, β-actin, and HRP-conjugated secondary antibodies (Cell Signaling Technology, Beverly, MA); pro-IL-1β, IL-1β, PGC-1α, TFAM, and SQSTM1 (Abcam, Cambridge, MA); MyHC (R&D Systems, Minneapolis, MN); Nlrp3 and ASC (AdipoGen, San Diego, CA); pro-IL-18 and IL-18 (MBL, Minneapolis, MN); and procaspase-1 and caspase-1 (Santa Cruz Biotechnology, Santa Cruz, CA). The SOD2 assay kit, MDA assay kit, and ATP assay kit (Beyotime Biotechnology, China) were also used.

Zhang Y., Liu Y., Bi X., Hu C., Ding F, & Ding W. (2019). Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise. Mediators of Inflammation, 2019, 2789014.

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