Brains including tumors from G-Smo pups were harvested, fixed in 4% paraformaldehyde for 48 hours, and embedded in paraffin at the UNC Center for Gastrointestinal Biology and Disease Histology core. Sections were deparaffinized, and antigen retrieval was performed using a low-pH citric acid–based buffer. Staining was performed, and stained slides were digitally scanned using the Leica Biosystems Aperio ImageScope software (12.3.3) with assistance from the UNC Translational Pathology Laboratory. The primary antibodies used were anti-pRB (catalog no. 8516, Cell Signaling Technology), anti-p4EBP1 (catalog no. 2855, Cell Signaling Technology), and anti-GLT1 (catalog no. 701988, Thermo Fisher Scientific).
Aperio imagescope software 12.3.3
Manufactured by Leica
Sourced in Germany
Aperio ImageScope software (12.3.3) is a digital pathology image viewer developed by Leica Biosystems. It provides basic functionalities for viewing and analyzing digital pathology slides.
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Lab products found in correlation
3 protocols using aperio imagescope software 12.3.3
1
Tumor Brain Histological Analysis
2
Brain Tissue Fixation and Staining
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Brains were fixed in 4% paraformaldehyde for at least 48 h. Tissue was processed and embedded in paraffin at the UNC Center for Gastrointestinal Biology and Disease Histology core. Sections were deparaffinized and antigen retrieval was performed using a low-pH citric acid-based buffer. Staining was performed with assistance from the Translational Pathology Laboratories. Slides were scanned using the Leica Biosystems Aperio ImageScope software (12.3.3).
3
Digitalization and Extraction of H&E Tissue Slides
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H&E tissue slides were digitalized using the Aperio AT2 microscopic slide scanner (Leica Biosystems, Wetzlar, Germany). H&E slides were scanned using either the 40× magnification (TMA slides) or the 20× magnification (whole-tissue slides). Microscopic image files were saved as Aperio ScanScope Virtual Slide (.SVS) files and displayed using Aperio ImageScope software 12.3.3 (Leica Biosystems, Wetzlar, Germany). Tissue spots were extracted from the TMAs using the Aperio Imagescope software. The images were resized to 50% of the pixel size with Image Resizer for Windows (version 3.1.1.) when scanned with a 40× magnification. In addition to tissue slides acquired as described above, we also obtained a previously described dataset composed of the following tissue type: adipose tissue (ADI, 10.407 images) [10 (link)].
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