Anti p62 ab56416

Manufactured by Abcam

Sourced in United Kingdom

Anti-p62 (ab56416) is a primary antibody that recognizes the p62 protein. p62, also known as SQSTM1, is a multifunctional protein involved in various cellular processes. This antibody can be used for the detection of p62 in various applications, such as Western blotting and immunohistochemistry.

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Lab products found in correlation

Chloroquine, by Merck Group (1 mentions) Recombinant human tgf β, by R&D Systems (1 mentions) Saracatinib, by AstraZeneca (1 mentions) Anti ctgf sc365970, by Santa Cruz Biotechnology (1 mentions) Anti phospho smad2 3 sc11769, by Santa Cruz Biotechnology (1 mentions) Haematoxylin, by Wuhan Servicebio Technology (1 mentions) Anti p ampk, by Cell Signaling Technology (1 mentions) Anti beclin1, by Abcam (1 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions) Nissl staining solution, by Wuhan Servicebio Technology (1 mentions) Anti neun, by Merck Group (1 mentions) Ecl chemiluminescence detection kit, by Wuhan Servicebio Technology (1 mentions) Anti lc3 14600 1 ap, by Proteintech (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions) Bca protein assay kit, by Applygen (1 mentions) Ripa buffer, by Applygen (1 mentions) Image pro express program, by Media Cybernetics (1 mentions) Bx51 microscope, by Olympus (1 mentions) Proteome profiler human phospho kinase array kit, by R&D Systems (1 mentions) Hpa002868, by Merck Group (1 mentions)

4 protocols using anti p62 ab56416

Saracatinib Modulation of TGF-β Signaling

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AstraZeneca UK Limited (London, UK) kindly provided the Src kinase inhibitor saracatinib (AZD0530). Recombinant human TGF-β (5 ng/mL) was purchased from R&D Systems (Minneapolis, MN, USA). Thioacetamide (TAA), PP2, and chloroquine were purchased from Sigma-Aldrich (St. Louis, MO, USA). PP2 was purchased from Millipore (Bedford, MA, USA). Anti-CTGF (SC-365970) and anti-phospho-Smad2/3 (SC11769) antibodies were purchased from Santa Cruz (Dallas, TX, USA). An anti-αSMA (A2547) antibody was purchased from Sigma-Aldrich. Anti-collagen (ab34710), anti-PAI-1 (ab66705), and anti-p62 (ab56416) antibodies were purchased from Abcam (Cambridge, UK). Anti-GAPDH (CS2118), anti-phospho-Src (Y416) (CS2101), anti-phospho-Smad3 (Ser423/425) (CS9520), anti-LC3B (CS2775), and anti-Atg7 (CS2631) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Seo H.Y., Lee S.H., Lee J.H., Kang Y.N., Hwang J.S., Park K.G., Kim M.K, & Jang B.K. (2020). Src Inhibition Attenuates Liver Fibrosis by Preventing Hepatic Stellate Cell Activation and Decreasing Connective Tissue Growth Factor. Cells, 9(3), 558.

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Icariin Modulates Autophagy in Neurons

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Icariin (ICA, purity >98.61%) was purchased from Cheng Du Purechem-Standard Co., Ltd. (Chengdu, China). Haematoxylin, eosin, Nissl staining solution and ECL chemiluminescence detection kit were purchased from Servicebio (Wuhan, China), RIPA buffer and BCA protein assay kit were purchased from Applygen (Beijing, China), PVDF was purchased from Millipore (MA, USA). Anti-LC3B (#83506), anti-p-ULK1 (ser757) (14202S), anti-p-mTOR (#5536), anti-p-AMPK (#2535) were purchased from Cell Signalling Technology (MA, USA). Anti-LC3 (14600-1-AP) was purchased from Proteintech (Wuhan, China). Anti-p62 (ab56416) and anti-Beclin1 were purchased from Abcam (Cambridge, UK). Anti-Atg5 (NB110-53818SS) was purchased from Novus Biologicals (CO, USA). Anti-NeuN (#2967854) was purchased from Millipore (MA, USA). Alexa Fluor 488 Donkey anti-Mouse IgG (H + L) (138499) and Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) (140019) were purchased from Jackson ImmunoResearch (PA, USA).

Zheng J., Hu S., Wang J., Zhang X., Yuan D., Zhang C., Liu C., Wang T, & Zhou Z. (2021). Icariin improves brain function decline in aging rats by enhancing neuronal autophagy through the AMPK/mTOR/ULK1 pathway. Pharmaceutical Biology, 59(1), 183-191.

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Immunostaining of Cellular Signaling Markers

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Fibroblasts and primary neurons were seeded on poly-lysine-coated coverslips (30,000 cells/cover). After treatment, cells were fixed in 4% paraformaldehyde/4% sucrose in PBS and permeabilized with 0.02% Triton X-100. Then, cells were blocked with 3% bovine serum albumin in PBS. Immunostaining was carried out using anti-tyrosine 412 phosphorylated of c-Abl (Y412) (anti-p-c-Abl) (C5240, Sigma Chemical co), anti-p62 (ab56416; Abcam), anti-LAMP1 (1D4B, sc-19992; Santa Cruz Biotechnology). Anti-rabbit IgG conjugated with Alexa Fluor-488 and anti-mouse IgG conjugated to Alexa Fluor-555 and Hoechst 33342 (H3570) were obtained from Invitrogen Detection Technologies. Fluorescent images were captured with an Olympus BX51 microscope (Olympus, Tokyo, Japan) and analyzed with the Image-Pro Express program (Media Cybernetics). We examined at least five images by cover and three covers by condition were stained by experiment in at least three independent experiments.

Marín T., Dulcey A.E., Campos F., de la Fuente C., Acuña M., Castro J., Pinto C., Yañez M.J., Cortez C., McGrath D.W., Sáez P.J., Gorshkov K., Zheng W., Southall N., Carmo-Fonseca M., Marugán J., Alvarez A.R, & Zanlungo S. (2022). c-Abl Activation Linked to Autophagy-Lysosomal Dysfunction Contributes to Neurological Impairment in Niemann-Pick Type A Disease. Frontiers in Cell and Developmental Biology, 10, 844297.

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Western Blot and Immunoprecipitation Assay for Cell Signaling

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The western blotting was performed as reported previously [37 (link)]. RIP3 immunoprecipitation was performed using the Pierce Co-Immunoprecipitation kit according to the manufacturer's instructions (Thermo Scientific, Inc., Pittsburgh, PA, USA). The primary antibodies included anti-cleaved caspase 3 (5A1E; 9664), anti-caspase 3 (8G10; 9665), anti-cleaved PARP (9544), anti-LC3A/B (4108), anti-LAMP1 (C54H11; 3243), anti-γH2A.X (2577), anti-RIP1 (D94C12; 3493), anti-histone 3 (D1H2; 4499; Cell Signaling Technology, Danvers, MA, USA), anti-p62 (ab56416, Abcam, Cambridge, UK), anti-ubiquitin (FK2; ST1200; Millipore, Billerica, MA, USA), anti-RIP3, anti-β-tubulin (D66; T0198), anti-RIP1 (334640; MAB3585, R&D Systems, Minneapolis, MN, USA), anti-RIP3 (R4277) and anti-SDHB (HPA002868; Sigma-Aldrich, St. Louis, MO, USA). For the detection of phospo-kinases, the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, Inc, Minneapolis, MN, USA) was used and the samples were analyzed according to the manufacturer's instructions.

Bullova P., Cougnoux A., Abunimer L., Kopacek J., Pastorekova S, & Pacak K. (2016). Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models. Oncotarget, 7(26), 40531-40545.

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