Cpa2250 electronic analytical balance

BL-420F organism functional experimental system (Chengdu Techman Co., Ltd., Chengdu, China) was used to record the lead II electrocardiograms (ECGs) of rats. CPA2250 electronic analytical balance (Sartorius, Germany) was used to correctly weigh the laboratory samples. HH-SJ heat-collecting magnetic stirrer (Jintan Chengdong Xinrui Instrument Factory, Changzhou, China) was used for simulating the process of sand frying by oil bath heating. Optical rotations were obtained on a PerkinElmer 341 polarimeter (PerkinElmer, USA). The melting point was measured on an X-4 micro-melting point apparatus and uncorrected (Shanghai Precision Scientific Instrument Corporation, Shanghai, China). NMR spectra were measured on a Bruker Avance 600 spectrometer (Bruker, Germany) in CDCl₃ with tetramethylsilane (TMS) as an internal standard. Mass spectra were carried out on a micrO-TOF-Q-II mass spectrometer (Bruker, Germany). RE-3000B rotary evaporator (Shanghai Yarong Biochemical Instrument Factory, Shanghai, China) was used to evaporate the solvent. Silica gel G (200–300 mesh) for column chromatography and TLC plates (silica gel G) were obtained from Qingdao Sea Chemical Factory (Qingdao, China). Spots on TLC plates were visualized with Dragendorff's reagent.

The lead II electrocardiograms (ECGs) were recorded using BL-420F Organism Functional Experimental System (Chengdu Techman Software Co., Ltd., Chengdu, China). CPA2250 electronic analytical balance (Sartorius, Germany) was used to correctly weigh the lab samples. The process of sand frying was simulated by oil bath heating using an HH-SJ heat-collecting magnetic stirrer (Jintan Chengdong Xinrui Instrument Factory, Changzhou, China). Optical rotations were obtained on a Perkin-Elmer 341 polarimeter (Perkin-Elmer, USA). Melting point was measured on an X-4 micro-melting point apparatus and uncorrected (Shanghai Precision Scientific Instrument Corporation, Shanghai, China). NMR spectra were measured on a Bruker Avance 600 spectrometer (Bruker, Germany) in CDCl₃ with tetramethylsilane (TMS) as the internal standard. Mass spectra were carried out on a micrOTOF-Q-II mass spectrometer (Bruker, Germany). Silica gel G (200–300 mesh) for column chromatography and TLC plates (silica gel G) were obtained from Qingdao Sea Chemical Factory (Qingdao, China). Spots on TLC plates were visualized with Dragendorff's reagent.