Na 201sx 4022

Manufactured by Olympus

Sourced in Japan

The NA-201SX-4022 is a laboratory equipment product made by Olympus. It is a compact and versatile device designed for general laboratory use. The core function of this product is to perform basic measurements and analysis tasks as required in various laboratory environments.

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34 protocols using na 201sx 4022

EBUS-TBNA Procedure for Lymph Node Evaluation

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EBUS‐TBNA procedures were performed by trained operators using a convex probe‐EBUS bronchoscope (BF‐UC260F‐OL8; Olympus) and a dedicated 22‐gauge needle (NA‐201SX‐4022; Olympus). The patients were under moderate sedation, achieved with intravenous midazolam and fentanyl.¹², ¹⁷ Lidocaine was used for local anesthesia. Hilar, interlobar, and lobar LNs recognized by real‐time ultrasound were examined by the operator. When possible, we conducted three passes per node. When core tissue was obtained, at least two passes were conducted when possible. However, when we obtained enough core tissue at the first pass and the patient's condition was unstable, we prematurely terminated the procedure after the first pass.
Once the tissue core had been secured, it was blotted in filter paper to remove excess blood, fixed in formalin, and then the tissue coagulum clot was sent for histological examination.¹⁸ Aspirate specimens were expelled onto glass slides, smeared, immediately fixed, and sent for cytological and/or histological examination.¹⁷ Rapid on‐site cytopathological evaluation was not performed.
Information on EBUS‐TBNA‐related adverse events (e.g., bleeding, hypoxemia, etc) was also collected.

Wi S., Kim B., Shin S.H., Jhun B.W., Yoo H., Jeong B., Lee K., Kim H., Kwon O.J., Han J., Kim J, & Um S. (2022). Clinical utility of EBUS‐TBNA of hilar, interlobar, and lobar lymph nodes in patients with primary lung cancer. Thoracic Cancer, 13(17), 2507-2514.

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EBUS-TBNA Procedure for Pulmonary Sampling

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All procedures were performed under general anesthesia using the rigid bronchoscope. EBUS-TBNA was performed through a dedicated endoscope (BF-UC180F, Olympus) using a convex probe with a 7.5 mHz transducer coupled on its tip which provides images parallel to the insertion of the bronchoscope. A 22-gauge needle (NA-201SX-4022, Olympus) was used to perform the aspirations.
The exams were carried out by the same three pulmonologists with about 2 years of experience in performing EBUS-TBNA, who had at least performed 40 or more procedures.

Cardoso A.V., Neves I., Magalhães A., Sucena M., Barroca H, & Fernandes G. (2015). The value of rapid on-site evaluation during EBUS-TBNA. Revista portuguesa de pneumologia, 21(5).

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EBUS-TBNA Paired Sampling for Molecular Analyses

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EBUS-TBNA was performed under conscious sedation as previously described using a dedicated linear array bronchoscope (BF-UC180F-OL8; Olympus, Tokyo, Japan) and 22-gauge needle (NA-201SX-4022; Olympus). 13, 14 For each patient, paired samples from the same target lymph node were obtained after tumor cells were demonstrated by rapid on-site evaluation as previously described. 15 The "reference" sample was the routine diagnostic specimen consisting of a minimum of 3 passes with 10 needle excursions per pass, and processed as a formalin-fixed paraffin-embedded (FFPE) cell block. The "study" sample comprised a single pass with 10 needle excursions per pass, and was snap frozen in dry ice and stored at À20 C before DNA extraction. A "pass" was defined as a distinct entry and exit of the needle into the target lesion. An "excursion" referred to a needle movement from the proximal to the distal side of the lesion prior to withdrawal of the needle from the lesion.

Leong T.L., Christie M., Kranz S., Pham K., Hsu A., Irving L.B., Asselin-Labat M.L, & Steinfort D.P. (2017). Evaluating the Genomic Yield of a Single Endobronchial Ultrasound-guided Transbronchial Needle Aspiration in Lung Cancer: Meeting the Challenge of Doing More With Less. Clinical lung cancer, 18(6).

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Bronchoscopic Techniques for Diagnosis and Staging

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An interventional pulmonologist or a thoracic surgeon performed all procedures. All procedures are performed either in endoscopy unit with moderate sedation or in the operation room (OR) under general anesthesia. We uniformly started bronchoscopies with airway examination by using a flexible bronchoscope (Evis Exera, BF-MP160F or BF-H190, Olympus, Tokyo, Japan) followed by EBUS examination (BF-UC180F convex probe EBUS ultrasound bronchoscope powered by Aloka ProSound F75 ultrasound processor, Olympus, Tokyo, Japan). A dedicated 22-gauge needle (NA-201SX-4022, Olympus) is used to perform transbronchial aspiration.

Berger J., Zamora F., Podgaetz E., Andrade R, & Dincer H.E. (2016). Usefulness of lymphoid granulomatous inflammation culture obtained by endobronchial ultrasound-guided transbronchial needle aspiration in a fungal endemic area. Endoscopic Ultrasound, 5(4), 243-247.

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EBUS-TBNA for Lymph Node Sampling

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Endobronchial ultrasonography was conducted using a fiberoptic ultrasound bronchoscope (Convex Probe EBUS; BF-UC 160F-OL8; Olympus Medical Systems, Tokyo, Japan). The location, shape, and structure of the lesions were examined with ultrasound. The locations of the stations were named and numbered using the lymph node map proposed by Mountain.[17 (link)] After the bronchoscope was guided to the target area, during real-time imaging, a 22-gauge aspirating needle with a syringe connected proximally (model NA-201SX-4022, Olympus, manufactured for this purpose) was pushed out from the distal tip of the bronchoscope, and samples consisting of cells or tissue fragments were obtained as previously described.[18 (link)] The aspirate was smeared onto glass slides, air-dried or fixed immediately with 95% alcohol, and stained with hematoxylin and eosin (H and E). Histological cores were fixed with 10% neutral buffered formalin and stained with H and E. Immunohistochemical staining was also performed when considered necessary. A rapid onsite cytopathological examination was not performed. Cytopathological specimens were categorized as (i) positive (adequate sample with the presence of malignant cells) or (ii) negative (sample consisting of mature lymphocytes and no malignant cells). Samples from all patients contained lymphocytes, and there were no inadequate samples by EBUS-TBNA.

Çetinkaya E., Usluer O., Yılmaz A., Tutar N., Çam E., Özgül M.A, & Demirci N.Y. (2017). Is endobronchial ultrasound-guided transbronchial needle aspiration an effective diagnostic procedure in restaging of non-small cell lung cancer patients?. Endoscopic Ultrasound, 6(3), 162-167.

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EBUS-TBNA and EUS-FNA-B/E for Lymph Node Evaluation

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EBUS-TBNA was performed using the standard methods described by our group in previous publications (10, 11) . Briefly, a routine endobronchial inspection was initially performed using a convex-probe ultrasound bronchoscope fitted with a linear transducer (BF-UC206F-OL8, Olympus, Tokyo, Japan). After the completion of EBUS-TBNA, the EBUS bronchoscope was re-inserted through the esophagus to assess the lymph nodes inaccessible by EBUS-TBNA; EUS-FNA-B/E was performed as needed (14) . TBNA or FNA biopsies were obtained using a dedicated 22-gauge needle (NA-201SX-4022, Olympus). All procedures were conducted with the patients under conscious sedation induced by midazolam and fentanyl. Nebulized lidocaine (4%) was used to achieve local anesthesia prior to each procedure. Bolus doses of 1.3% lidocaine were administered during the procedure, as needed. All aspirate specimens were smeared onto glass slides, fixed immediately, and sent for cytological and/or histological examination. Rapid on-site cytopathological evaluation (ROSE) was not performed.

Kang H.K., Um S.W., Jeong B.H., Lee K.J., Kim H., Kwon O.J, & Han J. (2016). The Utility of Endobronchial Ultrasound-guided Transbronchial Needle Aspiration in Patients with Small-cell Lung Cancer. Internal medicine (Tokyo, Japan), 55(9).

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Ultrasound-Guided EBUS-TBNA Procedure

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An expert pulmonologist (physician who has experienced EBUS-TBNA for over 10 years in department of respiratory medicine) performed the EBUS-TBNA procedures. Patients were placed in a conscious sedated state with midazolam. Lidocaine was administered for local anesthesia. A standard conventional flexible bronchoscopy (model BF-T160 bronchoscope, Olympus, Tokyo, Japan) was initially used to examine the tracheobronchial tree. A linear array ultrasonic bronchoscope (BF-UC260FW; Olympus, Tokyo, Japan) with a dedicated 22-gauge needle (NA-201SX-4022; Olympus, Tokyo, Japan) was subsequently used to perform all needle aspiration procedure. Lymph node or mass confirmed by imaging were located by ultrasound. TBNA was performed two or three times to obtain sample using real-time ultrasonic needle guidance through 2.2 mm working channel in the bronchoscope. When necessary, doppler ultrasound was used to identify vessels. TBNA was performed from distant lymph node/mass to avoid needle contamination by sampling highly suspected of metastasis on chest CT or ¹⁸F-FDG-PET-CT.

Moon K.M., Choi C.M., Ji W., Lee J.S., Lee S.W., Jo K.W., Song J.W, & Lee J.C. (2020). Clinical Characteristics of and Risk Factors for Fever after Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration: A Retrospective Study Involving 6336 Patients. Journal of Clinical Medicine, 9(1), 152.

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Optimized Transbronchial Biopsy and EBUS-TBNA

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Transbronchial biopsy was performed for primary lung lesions with radial probe EBUS (UM‐S20‐17S; Olympus) using the guide sheath method (EBUS‐GS‐TBB) (K‐201 guide sheath kit; Olympus). We routinely performed bronchial brushing and forceps biopsy five times.¹⁰, ¹³ During every bronchial brushing, the brush was directly suspended in physiological saline solution (PSS).
EBUS‐TBNA was performed to the hilar lymph nodes and mediastinal lymph nodes. We routinely performed three punctures with a 22G needle (NA‐201SX‐4022; Olympus) using an aspiration‐back‐lock syringe. After puncture, tissue samples were expelled by needle stylet. The tissue samples were fixed in formalin, and these tissue samples were not used for this study. The needle stylet was then removed, and PSS was injected into from needle tail (EBUS‐TBNA needle washing cytology sample). This procedure was repeated for every puncture.
The suspended solutions we obtained by bronchoscopy were immediately centrifugated, and only pellets were frozen and archived without DNA/RNA‐stabilizing solution in deep freeze. To maintain the quality and stability of DNA/RNA, all freezing and archiving procedures were carried out within 10 min from the time of sample collection. All cytology samples were pathologically confirmed to contain sufficient tumor cells (Figure 1).

Furuya N., Matsumoto S., Kakinuma K., Morikawa K., Inoue T., Saji H., Goto K, & Mineshita M. (2020). Suitability of transbronchial brushing cytology specimens for next‐generation sequencing in peripheral lung cancer. Cancer Science, 112(1), 380-387.

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EBUS-TBNA for Non-Squamous NSCLC

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All samples were obtained from EBUS-TBNA that was performed under moderate sedation as previously described [11] . A dedicated 22-gauge needle was used for aspiration (NA-201SX-4022; Olympus Ltd, Tokyo, Japan). Four to six needle passes per lymph node were suggested to be performed, three needle passes were permitted with satisfactory samples. Each pass included approximately 20 agitations of the needle within the target site. A single drop of sample material from each pass was deposited onto a glass slide, the remaining aspirate in the needle was expelled into formalin and was subsequently processed into a formalin-fixed paraffin-embedded (FFPE) cell block. All procedures were performed without rapid on-site evaluation (ROSE). Patients with a cell block diagnosed with non-squamous NSCLC according to histopathologic examination were finally enrolled in the study.

Xie F., Zheng X., Mao X., Zhao R., Ye J., Zhang Y, & Sun J. (2019). Next-Generation Sequencing for Genotyping of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Samples in Lung Cancer. The Annals of thoracic surgery, 108(1).

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EBUS-TBNA for Lymph Node Evaluation

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EBUS-TBNA was performed using a dedicated bronchoscope with a linear ultrasound transducer (BF-UC260F-OL8; Olympus, Tokyo, Japan). The target LN were punctured with a 21-gauge needle (NA-201SX-4022; Olympus, Tokyo, Japan). All patients provided written informed consent before undergoing the bronchoscopy. Two or more punctures were performed on each target lymph node to obtain at least two tissue core specimens. One of those specimens was prepared for histological examination, while the other specimens were utilized for AFB, Xpert, and MGIT960 culture. Ultrasonography was conducted with model HDI 5000, 7–12 MHz (Philips Medical Systems, Bothell, WA, USA) to evaluate the sizes (in cm) of superficial LN by measuring the largest diameters. Nodal masses with size > 5 mm were identified as bulky lesions. The size of LN was assessed by measuring the largest and smallest diameters on the ultrasound screen, and the long axis/short axis (L/S) ratio was calculated.

He X., Zhang Y., Zhou Y., Li L, & Li Q. (2023). Xpert MTB/RIF assay for the differential diagnosis between sarcoidosis and tuberculosis intrathoracic lymphadenopathy. BMC Infectious Diseases, 23, 725.

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