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β actin antibody a1978

Manufactured by Merck Group
Sourced in United States, China

The β-actin antibody (A1978) is a laboratory reagent used to detect the presence of the β-actin protein, which is a widely expressed cytoskeletal protein found in eukaryotic cells. This antibody can be used in various immunoassay techniques, such as Western blotting, to identify and quantify the β-actin protein in biological samples.

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6 protocols using β actin antibody a1978

1

Comprehensive Immune Marker Antibody Panel

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PBRM1 antibody (A301-591A) was from Bethyl Laboratories. Phospho-STAT1 antibody (clone ST1P-11A5; Tyr701; 33-3400), human CD3 antibody (clone F7.2.38; MA5-12577), human CD45RO antibody (clone UCHL1; MA5-11532), human CD4 antibody (clone 4B12; MS1528S0), and human CD8 antibody (clone C8/144B; MS457S0) were from ThermoFisher Scientific. Mouse CD3 antibody (D4V8L; 99940), mouse CD8 antibody(D4W22; 98941), mouse CD4 antibody (D7D2Z; 25229), human PD-L1 antibody (E1L3N; 13684), mouse PD-L1 antibody (D5V3B; 64988), mouse PD-1 antibody (D7D5W; 84651), PBRM1 antibody (D3F7O, 91894), JAK1 antibody (6G4, 3344), JAK2 antibody (D2E12, 3230), Phospho-JAK1 antibody (D7N4Z, Tyr1034/1035, 74129), Phospho-JAK2 antibody (C80C3, Tyr1007/1008, 3776), STAT1 antibody (D1K9Y, 14994), Phospho-STAT1 antibody (58D6, Tyr701, 9167), Phospho-STAT1 antibody (D3B7, Ser727, 8826), IRF1 antibody (D5E4, 8478), and BRG1 antibody (E9O6E; 52251) were from Cell Signaling Technology. IFNGR1 antibody (112802) was from BioLegend. IFNGR2 antibody (Cat No. GTX 64548) was from GeneTex. β-actin antibody (A1978) was from Sigma. Human IFNγ (285-IF-100) and mouse IFNγ (8234-MB-010) were from R&D Systems.
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2

Antibody Panel for Cell Signaling

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Tet1 (ab191698), Tet2 (ab94580), and CD146 (ab24577) antibodies were purchased from Abcam (Cambridge, MA, USA). Phospho-β-catenin (Ser552) and β-catenin (15B8) antibodies were purchased from Invitrogen (Carlsbad, CA, USA). FASL (SC-33716) and DKK-1 (SC-374574) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). β-Actin antibody (A1978) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-CD4-PerCP (550765) and anti-CD25-APC (561048) were purchased from BD Biosciences (San Jose, CA, USA). Anti-CD3 (100208) and anti-CD28 (102112) were purchased from BioLegend (SanDiego, CA, USA)
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3

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
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4

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
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5

Antibody and Inhibitor Protocols for Cell Research

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The antibody for FOXK2 (ab5298) was from Abcam Biotechnology company (Cambridge, United Kingdom). The β-Actin antibody (A1978) was from Sigma (St. Louis, MO) and mouse IgG (sc-2025) were from Santa Cruz Biotechnology (Dallas, TX). Doxorubicin (Dox, D1515), etoposide (VP16, E1383), 5-Fluorouracil (5FU, F6627), and Dichloroacetate (DCA) (347795) were from Sigma (St. Louis, MO). CDK2 inhibitors, Dinaciclib (HY-10492) and Alpelisib (BYL-719, HY-15244), were from MedChemExpress (Monmouth Junction, NJ).
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6

SARS-CoV-2 Nucleocapsid Protein Detection

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Antibody against tGFP (TA150041) was purchased from OriGene Technologies (Rockville, MD, USA), antibody against SARS-CoV-2 Nucleocapsid protein (40143-R019) was from Sino Biological (Beijing, China), and β-Actin antibody (A1978) was from Sigma-Aldrich (St. Louis, MO, USA). IRDye 680- or 800-labeled secondary antibodies were obtained from LI-COR Biosciences (Lincoln, NE, USA). E64-D (S7393) and remdesivir (S8932) were purchased from Selleck (Houston, TX, USA). E64-D and remdesivir were kept in DMSO at the concentration of 40 mg/mL and 10 mmol/L as stock solutions respectively.
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